##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AviII
AvrII BamHI BbeI BfrI BlnI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I FseI FspI HpaI KasI
MluI MroI NarI NheI NotI
NruI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2536 GGTACC G^GTACC
AccI 4119 GTCGAC GT^[AC][GT]AC
ApaI 48 GGGCCC GGGCC^C
AseI 5058 ATTAAT AT^TAAT
AsnI 5058 ATTAAT AT^TAAT
Asp700I 695 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2536 GGTACC G^GTACC
BcgI 3097 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3488 TGATCA T^GATCA
BspLU11I 799 ACATGT A^CATGT
BsrBI 3372 GAGCGG CCGCTC(-3/-3)
BstEII 3108 GGTGACC G^GT[ACGT]ACC
BstPI 3108 GGTGACC G^GT[ACGT]ACC
CfoI 1885 GCGC GCG^C
DraI 4892 TTTAAA TTT^AAA
DraIII 3671 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4601 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3108 GGTGACC G^GT[ACGT]ACC
EcoRV 2682 GATATC GAT^ATC
FbaI 3488 TGATCA T^GATCA
HaeII 1884 AGCGCC [GA]GCGC^[CT]
HgaI 4107 GACGC GACGC(5/10)
HhaI 1885 GCGC GCG^C
HinP1I 1885 GCGC G^CGC
HindIII 698 AAGCTT A^AGCTT
KpnI 2536 GGTACC GGTAC^C
NaeI 1569 GCCGGC GCC^GGC
NdeI 744 CATATG CA^TATG
NgoMIV 1569 GCCGGC G^CCGGC
PaeR7I 4573 CTCGAG C^TCGAG
PshBI 5058 ATTAAT AT^TAAT
SacI 3153 GAGCTC GAGCT^C
SalI 4119 GTCGAC G^TCGAC
SexAI 4812 ACCAGGT A^CC[AT]GGT
SphI 1207 GCATGC GCATG^C
SspI 720 AATATT AAT^ATT
XhoI 4573 CTCGAG C^TCGAG
XmnI 695 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 949 GACGTC GACGT^C
1146 GACGTC
AccII 2343 CGCG CG^CG
4617 CGCG
BalI 3125 TGGCCA TGG^CCA
3844 TGGCCA
BbrPI 2051 CACGTG CAC^GTG
3752 CACGTG
BbvCI 37 CCTCAGC CCTCAGC(-5/-2)
5125 CCTCAGC
BglII 773 AGATCT A^GATCT
2423 AGATCT
BlpI 4288 GCTCAGC GC^T[ACGT]AGC
4567 GCTCAGC
Bpu1102I 4288 GCTCAGC GC^T[ACGT]AGC
4567 GCTCAGC
BsaAI 2051 CACGTG [CT]AC^GT[GA]
3752 CACGTG
BsaBI 914 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3389 GATACAGATC
BsmBI 2630 CGTCTC CGTCTC(1/5)
1 GAGACG
BsrFI 1569 GCCGGC [GA]^CCGG[CT]
3525 ACCGGC
BstUI 2343 CGCG CG^CG
4617 CGCG
BstXI 4343 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4957 CCATCATCCTGG
CelII 4288 GCTCAGC GC^T[ACGT]AGC
4567 GCTCAGC
Cfr10I 1569 GCCGGC [GA]^CCGG[CT]
3525 ACCGGC
EcoRI 2773 GAATTC G^AATTC
5269 GAATTC
HincII 4119 GTCGAC GT[CT]^[GA]AC
5597 GTCAAC
HindII 4119 GTCGAC GT[CT]^[GA]AC
5597 GTCAAC
KspI 2342 CCGCGG CCGC^GG
4616 CCGCGG
MamI 914 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3389 GATACAGATC
MfeI 862 CAATTG C^AATTG
1051 CAATTG
MluNI 3125 TGGCCA TGG^CCA
3844 TGGCCA
MscI 3125 TGGCCA TGG^CCA
3844 TGGCCA
MunI 862 CAATTG C^AATTG
1051 CAATTG
MvnI 2343 CGCG CG^CG
4617 CGCG
PflMI 1331 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3043 CCACTGCCTGG
PmaCI 2051 CACGTG CAC^GTG
3752 CACGTG
PmlI 2051 CACGTG CAC^GTG
3752 CACGTG
SacII 2342 CCGCGG CCGC^GG
4616 CCGCGG
SapI 4828 GAAGAGC GCTCTTC(1/4)
5422 GCTCTTC
ScaI 2691 AGTACT AGT^ACT
3493 AGTACT
SmaI 354 CCCGGG CCC^GGG
3992 CCCGGG
Van91I 1331 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3043 CCACTGCCTGG
XbaI 1136 TCTAGA T^CTAGA
4589 TCTAGA
XmaCI 354 CCCGGG C^CCGGG
3992 CCCGGG
XmaI 354 CCCGGG C^CCGGG
3992 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII Hin1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII