##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
AscI Asp718I AspEI AvaI AviII
BclI BglI BsaWI BseAI BsoBI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I EcoRV
FbaI FseI FspI HindIII KpnI
MfeI MroI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RcaI RsrII SalI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 1970 CTTAAG C^TTAAG
Alw44I 4589 GTGCAC G^TGCAC
Aor51HI 4188 AGCGCT AGC^GCT
ApaLI 4589 GTGCAC G^TGCAC
AseI 5342 ATTAAT AT^TAAT
AsnI 5342 ATTAAT AT^TAAT
Asp700I 536 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 4551 GGCGCC GGCGC^C
BcgI 774 GCAGTACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1970 CTTAAG C^TTAAG
BsaBI 2251 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2519 CGTACG C^GTACG
BsmBI 2655 CGTCTC CGTCTC(1/5)
BsmI 2825 GCATTC GAATGC(1/-1)
BspLU11I 2928 ACATGT A^CATGT
BsrDI 2800 GCAATG GCAATG(2/0)
BstEII 3677 GGTCACC G^GT[ACGT]ACC
BstPI 3677 GGTCACC G^GT[ACGT]ACC
DraIII 606 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4188 AGCGCT AGC^GCT
EcoO65I 3677 GGTCACC G^GT[ACGT]ACC
EcoRI 2025 GAATTC G^AATTC
EcoT22I 2667 ATGCAT ATGCA^T
HpaI 2167 GTTAAC GTT^AAC
KasI 4551 GGCGCC G^GCGCC
KspI 2698 CCGCGG CCGC^GG
MamI 2251 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1692 ACGCGT A^CGCGT
NarI 4551 GGCGCC GG^CGCC
NsiI 2667 ATGCAT ATGCA^T
PshBI 5342 ATTAAT AT^TAAT
SacII 2698 CCGCGG CCGC^GG
SbfI 3939 CCTGCAGG CCTGCA^GG
SexAI 2058 ACCAGGT A^CC[AT]GGT
SfoI 4551 GGCGCC GGC^GCC
Sse8387I 3939 CCTGCAGG CCTGCA^GG
XbaI 326 TCTAGA T^CTAGA
XmnI 536 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 552 GTAGAC GT^[AC][GT]AC
3825 GTAGAC
AclI 1506 AACGTT AA^CGTT
2192 AACGTT
BalI 436 TGGCCA TGG^CCA
1586 TGGCCA
BamHI 1084 GGATCC G^GATCC
3779 GGATCC
BbrPI 663 CACGTG CAC^GTG
4586 CACGTG
BglII 2256 AGATCT A^GATCT
5289 AGATCT
BsiEI 4197 CGGCCG CG[GA][CT]^CG
4237 CGGCCG
BstAPI 1366 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4411 GCAGCCCCTGC
DsaI 2698 CCGCGG C^C[GA][CT]GG
4344 CCGTGG
EagI 4197 CGGCCG C^GGCCG
4237 CGGCCG
EclXI 4197 CGGCCG C^GGCCG
4237 CGGCCG
Eco52I 4197 CGGCCG C^GGCCG
4237 CGGCCG
HincII 2167 GTTAAC GT[CT]^[GA]AC
2315 GTCAAC
HindII 2167 GTTAAC GT[CT]^[GA]AC
2315 GTCAAC
MluNI 436 TGGCCA TGG^CCA
1586 TGGCCA
MscI 436 TGGCCA TGG^CCA
1586 TGGCCA
PmaCI 663 CACGTG CAC^GTG
4586 CACGTG
PmlI 663 CACGTG CAC^GTG
4586 CACGTG
Psp1406I 1506 AACGTT AA^CGTT
2192 AACGTT
SacI 2139 GAGCTC GAGCT^C
4474 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaII
AvrII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII