##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Aor51HI ApaI AscI Asp718I
AviII BamHI BanI BbeI BbrPI
BclI BcnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HpaII KasI KpnI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SapI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 2807 AACGTT AA^CGTT
AspI 4276 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3702 CTCGAG C^[CT]CG[GA]G
BcgI 7 CGAGCTTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1844 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5080 AGATCT A^GATCT
BlpI 2021 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2021 GCTCAGC GC^T[ACGT]AGC
BsaAI 2902 CACGTA [CT]AC^GT[GA]
BsmBI 1986 CGTCTC CGTCTC(1/5)
BsmI 1280 GCATTC GAATGC(1/-1)
BsoBI 3702 CTCGAG C^[CT]CG[GA]G
BspHI 61 TCATGA T^CATGA
BstAPI 1179 GCATGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 5080 AGATCT [GA]^GATC[CT]
CelII 2021 GCTCAGC GC^T[ACGT]AGC
DraIII 5210 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 4985 CCATGG C^C[GA][CT]GG
EcoRI 1139 GAATTC G^AATTC
HindIII 3159 AAGCTT A^AGCTT
HpaI 4538 GTTAAC GTT^AAC
MflI 5080 AGATCT [GA]^GATC[CT]
NcoI 4985 CCATGG C^CATGG
PaeR7I 3702 CTCGAG C^TCGAG
PflFI 4276 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 31 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2807 AACGTT AA^CGTT
RcaI 61 TCATGA T^CATGA
SbfI 1854 CCTGCAGG CCTGCA^GG
SexAI 528 ACCAGGT A^CC[AT]GGT
SfiI 1843 GGCCTGTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1854 CCTGCAGG CCTGCA^GG
StuI 3934 AGGCCT AGG^CCT
Tth111I 4276 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 31 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3702 CTCGAG C^TCGAG
XhoII 5080 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 3876 GTAGAC GT^[AC][GT]AC
4568 GTCTAC
AhdI 4697 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTTTCAGTC
Alw44I 1751 GTGCAC G^TGCAC
2366 GTGCAC
AocI 4217 CCTGAGG CC^T[ACGT]AGG
5070 CCTCAGG
ApaLI 1751 GTGCAC G^TGCAC
2366 GTGCAC
Asp700I 1412 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1570 GAATACATTC
AspEI 4697 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTTTCAGTC
BalI 321 TGGCCA TGG^CCA
976 TGGCCA
BanII 147 GAGCTC G[GA]GC[CT]^C
2826 GAGCTC
BbsI 1434 GAAGAC GAAGAC(2/6)
613 GTCTTC
BciVI 258 GGATAC GTATCC(6/5)
5132 GTATCC
BpuAI 1434 GAAGAC GAAGAC(2/6)
613 GTCTTC
BspLU11I 1654 ACATGT A^CATGT
3072 ACATGT
BssSI 5 CACGAG CACGAG(-5/-1)
726 CTCGTG
Bsu36I 4217 CCTGAGG CC^T[ACGT]AGG
5070 CCTCAGG
CfoI 95 GCGC GCG^C
2281 GCGC
EaeI 321 TGGCCA [CT]^GGCC[GA]
976 TGGCCA
Eam1105I 4697 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4926 GACTTTCAGTC
Eco81I 4217 CCTGAGG CC^T[ACGT]AGG
5070 CCTCAGG
HhaI 95 GCGC GCG^C
2281 GCGC
HinP1I 95 GCGC G^CGC
2281 GCGC
MfeI 2422 CAATTG C^AATTG
5146 CAATTG
MluNI 321 TGGCCA TGG^CCA
976 TGGCCA
MscI 321 TGGCCA TGG^CCA
976 TGGCCA
MspA1I 306 CAGCTG C[AC]G^C[GT]G
1944 CAGCTG
MunI 2422 CAATTG C^AATTG
5146 CAATTG
PpuMI 4635 GGGTCCT [GA]G^G[AT]CC[CT]
5090 GGGACCC
PvuII 306 CAGCTG CAG^CTG
1944 CAGCTG
SacI 147 GAGCTC GAGCT^C
2826 GAGCTC
ScaI 1017 AGTACT AGT^ACT
3709 AGTACT
SspI 3797 AATATT AAT^ATT
4317 AATATT
TaqI 298 TCGA T^CGA
3703 TCGA
TthHB8I 298 TCGA T^CGA
3703 TCGA
XbaI 968 TCTAGA T^CTAGA
3311 TCTAGA
XcmI 4665 CCATTTTAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4992 CCAGGAGGCATTTGG
XmnI 1412 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1570 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII AvrII BbvCI
BbvI BfaI BfrI BlnI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI