##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BciVI BglI BglII BlnI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I FseI FspI
HindIII HpaI KpnI KspI MamI
MluI MroI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfuI SphI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1959 GACGTC GACGT^C
AccII 3217 CGCG CG^CG
AflII 1570 CTTAAG C^TTAAG
AflIII 3859 ACATGT A^C[GA][CT]GT
BamHI 678 GGATCC G^GATCC
BbeI 2101 GGCGCC GGCGC^C
BbrPI 4411 CACGTG CAC^GTG
BbvCI 4322 GCTGAGG CCTCAGC(-5/-2)
BfrI 1570 CTTAAG C^TTAAG
BsaWI 3742 ACCGGA [AT]^CCGG[AT]
BsmBI 2094 GAGACG CGTCTC(1/5)
BspHI 2255 TCATGA T^CATGA
BspLU11I 3859 ACATGT A^CATGT
BstAPI 172 GCATGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3217 CGCG CG^CG
EcoT22I 404 ATGCAT ATGCA^T
KasI 2101 GGCGCC G^GCGCC
MfeI 801 CAATTG C^AATTG
MunI 801 CAATTG C^AATTG
MvnI 3217 CGCG CG^CG
NarI 2101 GGCGCC GG^CGCC
NcoI 2068 CCATGG C^CATGG
NheI 1439 GCTAGC G^CTAGC
NsiI 404 ATGCAT ATGCA^T
PmaCI 4411 CACGTG CAC^GTG
PmlI 4411 CACGTG CAC^GTG
PshAI 2918 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2255 TCATGA T^CATGA
SbfI 148 CCTGCAGG CCTGCA^GG
SexAI 1803 ACCTGGT A^CC[AT]GGT
SfoI 2101 GGCGCC GGC^GCC
SgrAI 2727 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 1584 TACGTA TAC^GTA
SpeI 4586 ACTAGT A^CTAGT
Sse8387I 148 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 3595 GTAGAC GT^[AC][GT]AC
4044 GTAGAC
ApaI 271 GGGCCC GGGCC^C
3005 GGGCCC
BclI 1862 TGATCA T^GATCA
2597 TGATCA
BlpI 1887 GCTGAGC GC^T[ACGT]AGC
3608 GCTGAGC
Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC
3608 GCTGAGC
BsaAI 1584 TACGTA [CT]AC^GT[GA]
4411 CACGTG
BsmI 910 GAATGC GAATGC(1/-1)
2464 GCATTC
Bsp1407I 2200 TGTACA T^GTACA
5219 TGTACA
BsrDI 36 GCAATG GCAATG(2/0)
583 GCAATG
BsrGI 2200 TGTACA T^GTACA
5219 TGTACA
CelII 1887 GCTGAGC GC^T[ACGT]AGC
3608 GCTGAGC
EcoRI 881 GAATTC G^AATTC
4648 GAATTC
EcoRV 3372 GATATC GAT^ATC
3450 GATATC
FbaI 1862 TGATCA T^GATCA
2597 TGATCA
SapI 2633 GAAGAGC GCTCTTC(1/4)
4629 GAAGAGC
SspBI 2200 TGTACA T^GTACA
5219 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI