##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AocI AscI
AseI AsnI Asp700I Asp718I AspI
BamHI BbvCI BciVI BclI BfrI
BglII BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BsmI BspEI
BspHI BspLU11I BsrBI Bst1107I BstEII
BstPI BstZ17I Bsu36I CelII CpoI
DraI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
KspI MamI MfeI MluI MroI
MseI MunI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AhdI 1634 GACCAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 956 GGGCCC GGGCC^C
AspEI 1634 GACCAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 110 CTCGGG C^[CT]CG[GA]G
AviII 147 TGCGCA TGC^GCA
AvrII 2120 CCTAGG C^CTAGG
BbeI 500 GGCGCC GGCGC^C
BbsI 1919 GTCTTC GAAGAC(2/6)
BlnI 2120 CCTAGG C^CTAGG
BpuAI 1919 GTCTTC GAAGAC(2/6)
BsoBI 110 CTCGGG C^[CT]CG[GA]G
BsrDI 1159 CATTGC GCAATG(2/0)
BsrFI 375 GCCGGC [GA]^CCGG[CT]
BssHII 483 GCGCGC G^CGCGC
BstAPI 1456 GCATGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 230 TTCGAA TT^CGAA
BstYI 1440 GGATCT [GA]^GATC[CT]
Cfr10I 375 GCCGGC [GA]^CCGG[CT]
DrdI 910 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1634 GACCAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 147 TGCGCA TGC^GCA
HincII 1061 GTCAAC GT[CT]^[GA]AC
HindII 1061 GTCAAC GT[CT]^[GA]AC
HindIII 2393 AAGCTT A^AGCTT
KasI 500 GGCGCC G^GCGCC
MflI 1440 GGATCT [GA]^GATC[CT]
NaeI 375 GCCGGC GCC^GGC
NarI 500 GGCGCC GG^CGCC
NdeI 1727 CATATG CA^TATG
NgoMIV 375 GCCGGC G^CCGGC
NspI 1456 GCATGC [GA]CATG^[CT]
NspV 230 TTCGAA TT^CGAA
PshAI 817 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 275 GAGCTC GAGCT^C
SexAI 1126 ACCTGGT A^CC[AT]GGT
SfoI 500 GGCGCC GGC^GCC
SfuI 230 TTCGAA TT^CGAA
SmlI 615 CTCAAG C^T[CT][GA]AG
SphI 1456 GCATGC GCATG^C
XbaI 2185 TCTAGA T^CTAGA
XcmI 504 CCAGCGTCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1440 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 52 GGCGTC G[GA]^CG[CT]C
500 GGCGCC
Alw44I 39 GTGCAC G^TGCAC
689 GTGCAC
Aor51HI 294 AGCGCT AGC^GCT
329 AGCGCT
ApaLI 39 GTGCAC G^TGCAC
689 GTGCAC
BbrPI 455 CACGTG CAC^GTG
604 CACGTG
BcgI 31 CGATGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
552 GCAGCTTCATCG
BglI 1691 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1852 GCCTGGGCGGC
BsaHI 52 GGCGTC G[GA]^CG[CT]C
500 GGCGCC
BsaI 1632 GAGACC GGTCTC(1/5)
2158 GAGACC
BsmAI 1632 GAGAC GTCTC(1/5)
2158 GAGAC
Bsp1407I 1521 TGTACA T^GTACA
1731 TGTACA
BspDI 29 ATCGAT AT^CGAT
1409 ATCGAT
BsrGI 1521 TGTACA T^GTACA
1731 TGTACA
ClaI 29 ATCGAT AT^CGAT
1409 ATCGAT
DraIII 692 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
704 CACACTGTG
Eco47III 294 AGCGCT AGC^GCT
329 AGCGCT
EcoNI 10 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1003 CCTGGAGAAGG
Hin1I 52 GGCGTC G[GA]^CG[CT]C
500 GGCGCC
NcoI 2042 CCATGG C^CATGG
2354 CCATGG
PmaCI 455 CACGTG CAC^GTG
604 CACGTG
PmlI 455 CACGTG CAC^GTG
604 CACGTG
SapI 226 GCTCTTC GCTCTTC(1/4)
463 GCTCTTC
SspBI 1521 TGTACA T^GTACA
1731 TGTACA
StuI 448 AGGCCT AGG^CCT
779 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I