##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AseI AsnI Asp718I
BfrI BglII BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI Bst1107I
BstAPI BstZ17I ClaI CpoI Eco47III
EcoRI EcoRV FseI HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NdeI NotI NruI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2920 GTCTAC GT^[AC][GT]AC
AhdI 1642 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 3531 GGCGCGCC GG^CGCGCC
AspEI 1642 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2129 TGCGCA TGC^GCA
AvrII 5032 CCTAGG C^CTAGG
BbeI 2388 GGCGCC GGCGC^C
BcgI 1102 CGAGGTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 788 GTATCC GTATCC(6/5)
BclI 3681 TGATCA T^GATCA
BlnI 5032 CCTAGG C^CTAGG
BsaBI 3570 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4917 ACATGT A^CATGT
BsrDI 3457 GCAATG GCAATG(2/0)
BssHII 3532 GCGCGC G^CGCGC
BssSI 412 CACGAG CACGAG(-5/-1)
BstEII 1807 GGTAACC G^GT[ACGT]ACC
BstPI 1807 GGTAACC G^GT[ACGT]ACC
Eam1105I 1642 GACCCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1807 GGTAACC G^GT[ACGT]ACC
FbaI 3681 TGATCA T^GATCA
FspI 2129 TGCGCA TGC^GCA
HincII 40 GTTGAC GT[CT]^[GA]AC
HindII 40 GTTGAC GT[CT]^[GA]AC
KasI 2388 GGCGCC G^GCGCC
MamI 3570 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2388 GGCGCC GG^CGCC
NheI 1080 GCTAGC G^CTAGC
ScaI 4346 AGTACT AGT^ACT
SfoI 2388 GGCGCC GGC^GCC
SmlI 162 CTCAAG C^T[CT][GA]AG
SspI 5122 AATATT AAT^ATT
StuI 4873 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BalI 2710 TGGCCA TGG^CCA
4773 TGGCCA
BamHI 640 GGATCC G^GATCC
1901 GGATCC
BsiEI 891 CGGCCG CG[GA][CT]^CG
1976 CGGCCG
Bsp1407I 4021 TGTACA T^GTACA
4332 TGTACA
BsrFI 889 GCCGGC [GA]^CCGG[CT]
4836 GCCGGC
BsrGI 4021 TGTACA T^GTACA
4332 TGTACA
BstBI 4962 TTCGAA TT^CGAA
4973 TTCGAA
Cfr10I 889 GCCGGC [GA]^CCGG[CT]
4836 GCCGGC
DrdI 706 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3463 GACAGATCGGTC
EagI 891 CGGCCG C^GGCCG
1976 CGGCCG
EclXI 891 CGGCCG C^GGCCG
1976 CGGCCG
Eco52I 891 CGGCCG C^GGCCG
1976 CGGCCG
EcoNI 1698 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4488 CCTAGCCAAGG
MluNI 2710 TGGCCA TGG^CCA
4773 TGGCCA
MscI 2710 TGGCCA TGG^CCA
4773 TGGCCA
NaeI 889 GCCGGC GCC^GGC
4836 GCCGGC
NgoMIV 889 GCCGGC G^CCGGC
4836 GCCGGC
NspV 4962 TTCGAA TT^CGAA
4973 TTCGAA
SfuI 4962 TTCGAA TT^CGAA
4973 TTCGAA
SmaI 2601 CCCGGG CCC^GGG
3312 CCCGGG
SspBI 4021 TGTACA T^GTACA
4332 TGTACA
XbaI 625 TCTAGA T^CTAGA
3858 TCTAGA
XmaCI 2601 CCCGGG C^CCGGG
3312 CCCGGG
XmaI 2601 CCCGGG C^CCGGG
3312 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AspI AvaI AvaII BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI