##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
Asp700I AviII BfrI BsaBI BseAI
BsiWI BspDI BspEI BsrFI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII EcoRV FseI FspI HincII
HindII HpaI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2543 GACGTC GACGT^C
Acc65I 4255 GGTACC G^GTACC
AccI 421 GTCTAC GT^[AC][GT]AC
Aor51HI 5313 AGCGCT AGC^GCT
AseI 2974 ATTAAT AT^TAAT
AsnI 2974 ATTAAT AT^TAAT
Asp718I 4255 GGTACC G^GTACC
BamHI 2505 GGATCC G^GATCC
BcgI 5081 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2827 GTATCC GTATCC(6/5)
BclI 5370 TGATCA T^GATCA
BglII 602 AGATCT A^GATCT
BsaWI 2735 TCCGGT [AT]^CCGG[AT]
BsmI 1872 GAATGC GAATGC(1/-1)
BspHI 2044 TCATGA T^CATGA
BspLU11I 2379 ACATGT A^CATGT
BsrBI 3985 GAGCGG CCGCTC(-3/-3)
BssHII 5046 GCGCGC G^CGCGC
DraI 4881 TTTAAA TTT^AAA
Eco47III 5313 AGCGCT AGC^GCT
FbaI 5370 TGATCA T^GATCA
HindIII 2126 AAGCTT A^AGCTT
KpnI 4255 GGTACC GGTAC^C
PaeR7I 638 CTCGAG C^TCGAG
PshAI 414 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2974 ATTAAT AT^TAAT
RcaI 2044 TCATGA T^CATGA
SexAI 132 ACCAGGT A^CC[AT]GGT
SfiI 4090 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XhoI 638 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 1902 GTGCAC G^TGCAC
2220 GTGCAC
ApaLI 1902 GTGCAC G^TGCAC
2220 GTGCAC
AspI 5142 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5816 GACTCTGTC
BbeI 3393 GGCGCC GGCGC^C
4594 GGCGCC
BsiEI 2255 CGGCCG CG[GA][CT]^CG
3363 CGGCCG
BstEII 855 GGTCACC G^GT[ACGT]ACC
3961 GGTCACC
BstPI 855 GGTCACC G^GT[ACGT]ACC
3961 GGTCACC
DrdI 217 GACCCATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
252 GACCCTCAGGTC
EagI 2255 CGGCCG C^GGCCG
3363 CGGCCG
EclXI 2255 CGGCCG C^GGCCG
3363 CGGCCG
Eco52I 2255 CGGCCG C^GGCCG
3363 CGGCCG
EcoO65I 855 GGTCACC G^GT[ACGT]ACC
3961 GGTCACC
EcoT22I 1874 ATGCAT ATGCA^T
5486 ATGCAT
KasI 3393 GGCGCC G^GCGCC
4594 GGCGCC
NarI 3393 GGCGCC GG^CGCC
4594 GGCGCC
NsiI 1874 ATGCAT ATGCA^T
5486 ATGCAT
PflFI 5142 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5816 GACTCTGTC
SbfI 980 CCTGCAGG CCTGCA^GG
1736 CCTGCAGG
SfoI 3393 GGCGCC GGC^GCC
4594 GGCGCC
Sse8387I 980 CCTGCAGG CCTGCA^GG
1736 CCTGCAGG
TaqI 639 TCGA T^CGA
2061 TCGA
Tth111I 5142 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5816 GACTCTGTC
TthHB8I 639 TCGA T^CGA
2061 TCGA
XbaI 3153 TCTAGA T^CTAGA
4381 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI ApoI AspEI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI