##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI Asp718I BbrPI
BclI BfrI BsaBI BspDI BspLU11I
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraIII EagI
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MluI NotI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RsrII SacII SalI SbfI
SfiI SgrAI SpeI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 3690 ACACGT A^C[GA][CT]GT
Aor51HI 499 AGCGCT AGC^GCT
ApaI 2000 GGGCCC GGGCC^C
AseI 3653 ATTAAT AT^TAAT
AsnI 3653 ATTAAT AT^TAAT
AspI 1803 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2742 CCTAGG C^CTAGG
BglI 3713 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4971 AGATCT A^GATCT
BlnI 2742 CCTAGG C^CTAGG
BsiEI 3230 CGATCG CG[GA][CT]^CG
BsiWI 2444 CGTACG C^GTACG
BsmBI 2993 CGTCTC CGTCTC(1/5)
BspHI 4677 TCATGA T^CATGA
BssSI 1271 CTCGTG CACGAG(-5/-1)
BstBI 210 TTCGAA TT^CGAA
DraI 1716 TTTAAA TTT^AAA
DrdI 3030 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 499 AGCGCT AGC^GCT
EcoRI 4080 GAATTC G^AATTC
MfeI 3046 CAATTG C^AATTG
MunI 3046 CAATTG C^AATTG
NdeI 3531 CATATG CA^TATG
NheI 502 GCTAGC G^CTAGC
NruI 3127 TCGCGA TCG^CGA
NspV 210 TTCGAA TT^CGAA
PflFI 1803 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3653 ATTAAT AT^TAAT
PvuI 3230 CGATCG CGAT^CG
RcaI 4677 TCATGA T^CATGA
SacI 2346 GAGCTC GAGCT^C
SexAI 171 ACCTGGT A^CC[AT]GGT
SfuI 210 TTCGAA TT^CGAA
SmaI 2523 CCCGGG CCC^GGG
SnaBI 2446 TACGTA TAC^GTA
SphI 3181 GCATGC GCATG^C
Tth111I 1803 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2523 CCCGGG C^CCGGG
XmaI 2523 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2287 GTCTAC GT^[AC][GT]AC
2710 GTCTAC
AhdI 4619 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4847 GACTACGAGTC
AocI 3445 CCTTAGG CC^T[ACGT]AGG
4445 CCTGAGG
AspEI 4619 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4847 GACTACGAGTC
AviII 1670 TGCGCA TGC^GCA
2355 TGCGCA
BamHI 1079 GGATCC G^GATCC
4876 GGATCC
BciVI 2430 GGATAC GTATCC(6/5)
3694 GTATCC
BsmI 4057 GAATGC GAATGC(1/-1)
4732 GAATGC
Bsp1407I 1275 TGTACA T^GTACA
3687 TGTACA
BsrGI 1275 TGTACA T^GTACA
3687 TGTACA
Bsu36I 3445 CCTTAGG CC^T[ACGT]AGG
4445 CCTGAGG
Eam1105I 4619 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4847 GACTACGAGTC
Eco81I 3445 CCTTAGG CC^T[ACGT]AGG
4445 CCTGAGG
FspI 1670 TGCGCA TGC^GCA
2355 TGCGCA
HincII 2897 GTTGAC GT[CT]^[GA]AC
3465 GTCAAC
HindII 2897 GTTGAC GT[CT]^[GA]AC
3465 GTCAAC
HindIII 539 AAGCTT A^AGCTT
3471 AAGCTT
NaeI 3062 GCCGGC GCC^GGC
3833 GCCGGC
NcoI 682 CCATGG C^CATGG
2582 CCATGG
NgoMIV 3062 GCCGGC G^CCGGC
3833 GCCGGC
NspI 3181 GCATGC [GA]CATG^[CT]
4942 GCATGT
PaeR7I 357 CTCGAG C^TCGAG
1529 CTCGAG
PstI 513 CTGCAG CTGCA^G
1462 CTGCAG
SspBI 1275 TGTACA T^GTACA
3687 TGTACA
StuI 1011 AGGCCT AGG^CCT
4215 AGGCCT
XbaI 2300 TCTAGA T^CTAGA
4195 TCTAGA
XhoI 357 CTCGAG C^TCGAG
1529 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI