##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AgeI Aor51HI AseI AsnI AviII
BbvCI BclI BglII BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
CelII CpoI DraI Eco47III EcoRV
FbaI FseI FspI HindIII KspI
MamI MfeI MluI MunI NdeI
NotI NruI NspV PacI PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacII ScaI SfuI SnaBI
SphI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 5 GACGTC GACGT^C
AccIII 1593 TCCGGA T^CCGGA
AclI 4530 AACGTT AA^CGTT
AflII 3846 CTTAAG C^TTAAG
AhdI 4548 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4022 GTGCAC G^TGCAC
ApaLI 4022 GTGCAC G^TGCAC
AscI 3274 GGCGCGCC GG^CGCGCC
Asp700I 4248 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4548 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 467 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4704 CCTAGG C^CTAGG
BamHI 2694 GGATCC G^GATCC
BfrI 3846 CTTAAG C^TTAAG
BlnI 4704 CCTAGG C^CTAGG
BseAI 1593 TCCGGA T^CCGGA
BsmI 5175 GCATTC GAATGC(1/-1)
BspEI 1593 TCCGGA T^CCGGA
BssHII 3275 GCGCGC G^CGCGC
BstYI 2694 GGATCC [GA]^GATC[CT]
DrdI 464 GACGACTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2520 CGGCCG C^GGCCG
Eam1105I 4548 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2520 CGGCCG C^GGCCG
Eco52I 2520 CGGCCG C^GGCCG
EcoRI 4252 GAATTC G^AATTC
EcoT22I 1773 ATGCAT ATGCA^T
HpaI 4451 GTTAAC GTT^AAC
MflI 2694 GGATCC [GA]^GATC[CT]
MroI 1593 TCCGGA T^CCGGA
NaeI 2522 GCCGGC GCC^GGC
NcoI 4665 CCATGG C^CATGG
NgoMIV 2522 GCCGGC G^CCGGC
NheI 2660 GCTAGC G^CTAGC
NsiI 1773 ATGCAT ATGCA^T
PaeR7I 3446 CTCGAG C^TCGAG
PflFI 467 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4530 AACGTT AA^CGTT
SalI 4427 GTCGAC G^TCGAC
SapI 2323 GCTCTTC GCTCTTC(1/4)
SgrAI 1676 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 350 CCCGGG CCC^GGG
SpeI 4447 ACTAGT A^CTAGT
Tth111I 467 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3998 TCTAGA T^CTAGA
XhoI 3446 CTCGAG C^TCGAG
XhoII 2694 GGATCC [GA]^GATC[CT]
XmaCI 350 CCCGGG C^CCGGG
XmaI 350 CCCGGG C^CCGGG
XmnI 4248 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 3740 GTCTAC GT^[AC][GT]AC
4427 GTCGAC
AlwI 2695 GATCC GGATC(4/5)
2694 GGATC
BbeI 1052 GGCGCC GGCGC^C
1961 GGCGCC
BciVI 740 GGATAC GTATCC(6/5)
288 GTATCC
BsaWI 746 ACCGGA [AT]^CCGG[AT]
1593 TCCGGA
BsiEI 2520 CGGCCG CG[GA][CT]^CG
5112 CGACCG
BsmBI 2443 CGTCTC CGTCTC(1/5)
4313 GAGACG
BspDI 371 ATCGAT AT^CGAT
1868 ATCGAT
BsrBI 2491 CCGCTC CCGCTC(-3/-3)
2143 GAGCGG
BstEII 2434 GGTCACC G^GT[ACGT]ACC
2644 GGTCACC
BstPI 2434 GGTCACC G^GT[ACGT]ACC
2644 GGTCACC
ClaI 371 ATCGAT AT^CGAT
1868 ATCGAT
EcoO65I 2434 GGTCACC G^GT[ACGT]ACC
2644 GGTCACC
EcoT14I 4665 CCATGG C^C[AT][AT]GG
4704 CCTAGG
KasI 1052 GGCGCC G^GCGCC
1961 GGCGCC
NarI 1052 GGCGCC GG^CGCC
1961 GGCGCC
NspI 1775 GCATGT [GA]CATG^[CT]
4592 GCATGT
SbfI 2022 CCTGCAGG CCTGCA^GG
2060 CCTGCAGG
SexAI 65 ACCTGGT A^CC[AT]GGT
4342 ACCAGGT
SfoI 1052 GGCGCC GGC^GCC
1961 GGCGCC
Sse8387I 2022 CCTGCAGG CCTGCA^GG
2060 CCTGCAGG
StyI 4665 CCATGG C^C[AT][AT]GG
4704 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwNI AocI
ApaI ApoI Asp718I AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI