##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AseI AsnI AspEI AviII
BfrI BsaBI BsiWI BsmI BspDI
BstBI ClaI CpoI Eam1105I Eco47III
EcoRV FseI FspI HpaI KspI
MamI MfeI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII ScaI SfiI SfuI
SgrAI SpeI SphI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1525 GACGTC GACGT^C
Acc65I 4976 GGTACC G^GTACC
AccIII 3931 TCCGGA T^CCGGA
AclI 3104 AACGTT AA^CGTT
AscI 2571 GGCGCGCC GG^CGCGCC
Asp700I 297 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4976 GGTACC G^GTACC
AspI 3171 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 512 GGATCC G^GATCC
BbrPI 4817 CACGTG CAC^GTG
BciVI 1858 GGATAC GTATCC(6/5)
BclI 41 TGATCA T^GATCA
BglII 2708 AGATCT A^GATCT
BsaWI 3931 TCCGGA [AT]^CCGG[AT]
BseAI 3931 TCCGGA T^CCGGA
BsiEI 4843 CGGCCG CG[GA][CT]^CG
BsmBI 235 GAGACG CGTCTC(1/5)
Bsp1407I 1752 TGTACA T^GTACA
BspEI 3931 TCCGGA T^CCGGA
BspLU11I 1822 ACATGT A^CATGT
BsrBI 2146 GAGCGG CCGCTC(-3/-3)
BsrDI 3454 CATTGC GCAATG(2/0)
BsrGI 1752 TGTACA T^GTACA
Bst1107I 2488 GTATAC GTA^TAC
BstZ17I 2488 GTATAC GTA^TAC
DrdI 3543 GACTTAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4843 CGGCCG C^GGCCG
EclXI 4843 CGGCCG C^GGCCG
Eco52I 4843 CGGCCG C^GGCCG
EcoT22I 1615 ATGCAT ATGCA^T
FbaI 41 TGATCA T^GATCA
HindIII 2411 AAGCTT A^AGCTT
KpnI 4976 GGTACC GGTAC^C
MluI 4692 ACGCGT A^CGCGT
MroI 3931 TCCGGA T^CCGGA
NaeI 4375 GCCGGC GCC^GGC
NgoMIV 4375 GCCGGC G^CCGGC
NsiI 1615 ATGCAT ATGCA^T
PflFI 3171 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4817 CACGTG CAC^GTG
PmlI 4817 CACGTG CAC^GTG
Psp1406I 3104 AACGTT AA^CGTT
SalI 4164 GTCGAC G^TCGAC
SexAI 4734 ACCTGGT A^CC[AT]GGT
SnaBI 2491 TACGTA TAC^GTA
SspBI 1752 TGTACA T^GTACA
SspI 3511 AATATT AAT^ATT
Tth111I 3171 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4116 CCAGCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 297 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BcgI 599 CGAGGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4794 GCAAGGGCTTCG
BspHI 2456 TCATGA T^CATGA
3054 TCATGA
BsrFI 4375 GCCGGC [GA]^CCGG[CT]
4533 ACCGGC
BssHII 2572 GCGCGC G^CGCGC
4860 GCGCGC
BstEII 727 GGTGACC G^GT[ACGT]ACC
1278 GGTGACC
BstPI 727 GGTGACC G^GT[ACGT]ACC
1278 GGTGACC
Cfr10I 4375 GCCGGC [GA]^CCGG[CT]
4533 ACCGGC
DraI 1852 TTTAAA TTT^AAA
3654 TTTAAA
EcoO65I 727 GGTGACC G^GT[ACGT]ACC
1278 GGTGACC
EcoRI 3568 GAATTC G^AATTC
5405 GAATTC
HincII 2764 GTCAAC GT[CT]^[GA]AC
4164 GTCGAC
HindII 2764 GTCAAC GT[CT]^[GA]AC
4164 GTCGAC
PflMI 1536 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5470 CCAGGGCCTGG
PshAI 653 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
737 GACGCATGTC
RcaI 2456 TCATGA T^CATGA
3054 TCATGA
SbfI 1046 CCTGCAGG CCTGCA^GG
1374 CCTGCAGG
Sse8387I 1046 CCTGCAGG CCTGCA^GG
1374 CCTGCAGG
Van91I 1536 CCAAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5470 CCAGGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI