##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AviII BbeI BclI
BfrI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I FbaI FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2031 GGTACC G^GTACC
AcyI 3164 GGCGTC G[GA]^CG[CT]C
AocI 2864 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1042 AGCGCT AGC^GCT
Asp700I 2192 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2031 GGTACC G^GTACC
AspI 5402 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1599 CCTAGG C^CTAGG
BbrPI 282 CACGTG CAC^GTG
BcgI 3159 GCATTGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1599 CCTAGG C^CTAGG
BsaAI 282 CACGTG [CT]AC^GT[GA]
BsaHI 3164 GGCGTC G[GA]^CG[CT]C
BsgI 523 GTGCAG GTGCAG(16/14)
BsmI 275 GCATTC GAATGC(1/-1)
BspHI 1533 TCATGA T^CATGA
BspLU11I 1169 ACATGT A^CATGT
BsrBI 1865 GAGCGG CCGCTC(-3/-3)
BsrFI 3430 GCCGGT [GA]^CCGG[CT]
BstAPI 2288 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2864 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3430 GCCGGT [GA]^CCGG[CT]
DraI 5418 TTTAAA TTT^AAA
DraIII 4495 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1042 AGCGCT AGC^GCT
Eco81I 2864 CCTGAGG CC^T[ACGT]AGG
EcoRI 1072 GAATTC G^AATTC
EcoRV 4424 GATATC GAT^ATC
EcoT22I 5250 ATGCAT ATGCA^T
Hin1I 3164 GGCGTC G[GA]^CG[CT]C
HindIII 592 AAGCTT A^AGCTT
KpnI 2031 GGTACC GGTAC^C
NdeI 2857 CATATG CA^TATG
NheI 2900 GCTAGC G^CTAGC
NsiI 5250 ATGCAT ATGCA^T
PflFI 5402 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 282 CACGTG CAC^GTG
PmlI 282 CACGTG CAC^GTG
PshAI 1657 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1533 TCATGA T^CATGA
SapI 38 GCTCTTC GCTCTTC(1/4)
SexAI 975 ACCAGGT A^CC[AT]GGT
SfiI 3686 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1941 ACTAGT A^CTAGT
Tth111I 5402 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4686 TCTAGA T^CTAGA
XmnI 2192 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 3492 GTCTAC GT^[AC][GT]AC
4876 GTAGAC
AccII 220 CGCG CG^CG
3266 CGCG
AflIII 281 ACACGT A^C[GA][CT]GT
1169 ACATGT
ApaI 2640 GGGCCC GGGCC^C
3685 GGGCCC
BamHI 343 GGATCC G^GATCC
4386 GGATCC
BanI 4 GGTGCC G^G[CT][GA]CC
2031 GGTACC
BglI 3150 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3687 GCCCTCATGGC
BglII 2525 AGATCT A^GATCT
4285 AGATCT
BsmBI 3192 CGTCTC CGTCTC(1/5)
5407 CGTCTC
Bsp1407I 2892 TGTACA T^GTACA
5111 TGTACA
BspMI 1855 GCAGGT ACCTGC(4/8)
4916 GCAGGT
BsrGI 2892 TGTACA T^GTACA
5111 TGTACA
BstUI 220 CGCG CG^CG
3266 CGCG
HgaI 1320 GCGTC GACGC(5/10)
3165 GCGTC
HincII 390 GTTGAC GT[CT]^[GA]AC
1654 GTTGAC
HindII 390 GTTGAC GT[CT]^[GA]AC
1654 GTTGAC
MvnI 220 CGCG CG^CG
3266 CGCG
SphI 3551 GCATGC GCATG^C
3752 GCATGC
SspBI 2892 TGTACA T^GTACA
5111 TGTACA
SspI 1264 AATATT AAT^ATT
4562 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII