##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AclI AflIII AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII BamHI BbrPI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsmI
BspDI BspLU11I BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HpaI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SexAI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2307 GGTACC G^GTACC
AflII 667 CTTAAG C^TTAAG
AocI 977 CCTCAGG CC^T[ACGT]AGG
Asp718I 2307 GGTACC G^GTACC
AspI 1826 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2527 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3325 GGATAC GTATCC(6/5)
BclI 4358 TGATCA T^GATCA
BfrI 667 CTTAAG C^TTAAG
BglII 5030 AGATCT A^GATCT
BsaAI 1679 TACGTG [CT]AC^GT[GA]
BsmBI 4699 GAGACG CGTCTC(1/5)
Bsp1407I 1236 TGTACA T^GTACA
BsrGI 1236 TGTACA T^GTACA
BstEII 3193 GGTAACC G^GT[ACGT]ACC
BstPI 3193 GGTAACC G^GT[ACGT]ACC
Bsu36I 977 CCTCAGG CC^T[ACGT]AGG
DraI 5145 TTTAAA TTT^AAA
DraIII 4789 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 977 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3193 GGTAACC G^GT[ACGT]ACC
EcoRI 4687 GAATTC G^AATTC
FbaI 4358 TGATCA T^GATCA
KpnI 2307 GGTACC GGTAC^C
KspI 4735 CCGCGG CCGC^GG
NcoI 4034 CCATGG C^CATGG
NheI 3996 GCTAGC G^CTAGC
PaeR7I 2191 CTCGAG C^TCGAG
PflFI 1826 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 4735 CCGCGG CCGC^GG
SfiI 2592 GGCCTCACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2842 CCCGGG CCC^GGG
SphI 411 GCATGC GCATG^C
SspBI 1236 TGTACA T^GTACA
SspI 1367 AATATT AAT^ATT
Tth111I 1826 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2191 CTCGAG C^TCGAG
XmaCI 2842 CCCGGG C^CCGGG
XmaI 2842 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 3118 AAATTT [GA]^AATT[CT]
4687 GAATTC
AgeI 1809 ACCGGT A^CCGGT
2304 ACCGGT
ApoI 3118 AAATTT [GA]^AATT[CT]
4687 GAATTC
BsrBI 523 CCGCTC CCGCTC(-3/-3)
326 GAGCGG
BsrDI 1924 CATTGC GCAATG(2/0)
2433 GCAATG
BssHII 53 GCGCGC G^CGCGC
55 GCGCGC
BstYI 4107 GGATCT [GA]^GATC[CT]
5030 AGATCT
HincII 3074 GTCAAC GT[CT]^[GA]AC
4495 GTCAAC
HindII 3074 GTCAAC GT[CT]^[GA]AC
4495 GTCAAC
HindIII 2468 AAGCTT A^AGCTT
4650 AAGCTT
MflI 4107 GGATCT [GA]^GATC[CT]
5030 AGATCT
PinAI 1809 ACCGGT A^CCGGT
2304 ACCGGT
SacI 614 GAGCTC GAGCT^C
1034 GAGCTC
SapI 700 GAAGAGC GCTCTTC(1/4)
1981 GCTCTTC
ScaI 459 AGTACT AGT^ACT
4409 AGTACT
XcmI 2934 CCAAGCCCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3263 CCAGCAGCTGGCTGG
XhoII 4107 GGATCT [GA]^GATC[CT]
5030 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AvrII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspHI
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfoI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I