##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BcgI
BciVI BfrI BlnI BsaBI BsiWI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Cfr10I
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoO65I EcoT22I FseI FspI
KasI KpnI MamI MluI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SbfI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 3750 TCCGGA T^CCGGA
AcyI 3903 GGCGTC G[GA]^CG[CT]C
Aor51HI 1345 AGCGCT AGC^GCT
ApaI 178 GGGCCC GGGCC^C
AseI 2513 ATTAAT AT^TAAT
AsnI 2513 ATTAAT AT^TAAT
AspI 3110 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3133 CCTCAGC CCTCAGC(-5/-2)
BclI 4957 TGATCA T^GATCA
BglII 2845 AGATCT A^GATCT
BsaAI 1883 TACGTG [CT]AC^GT[GA]
BsaHI 3903 GGCGTC G[GA]^CG[CT]C
BseAI 3750 TCCGGA T^CCGGA
BsiEI 12 CGGTCG CG[GA][CT]^CG
BsmBI 1006 GAGACG CGTCTC(1/5)
Bsp1407I 1666 TGTACA T^GTACA
BspDI 2323 ATCGAT AT^CGAT
BspEI 3750 TCCGGA T^CCGGA
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrGI 1666 TGTACA T^GTACA
BstBI 807 TTCGAA TT^CGAA
ClaI 2323 ATCGAT AT^CGAT
DraIII 3020 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 68 CCGCGG C^C[GA][CT]GG
Eco47III 1345 AGCGCT AGC^GCT
EcoRI 3914 GAATTC G^AATTC
EcoRV 388 GATATC GAT^ATC
FauI 105 CCCGC CCCGC(4/6)
FbaI 4957 TGATCA T^GATCA
Hin1I 3903 GGCGTC G[GA]^CG[CT]C
HpaI 4354 GTTAAC GTT^AAC
KspI 68 CCGCGG CCGC^GG
MfeI 4772 CAATTG C^AATTG
MroI 3750 TCCGGA T^CCGGA
MunI 4772 CAATTG C^AATTG
NspV 807 TTCGAA TT^CGAA
PaeR7I 411 CTCGAG C^TCGAG
PflFI 3110 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 154 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2513 ATTAAT AT^TAAT
SacII 68 CCGCGG CCGC^GG
SalI 2103 GTCGAC G^TCGAC
ScaI 4115 AGTACT AGT^ACT
SfuI 807 TTCGAA TT^CGAA
SphI 4144 GCATGC GCATG^C
SspBI 1666 TGTACA T^GTACA
Tth111I 3110 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3348 CCAACAACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 411 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2103 GTCGAC GT^[AC][GT]AC
3393 GTCTAC
Alw44I 1886 GTGCAC G^TGCAC
2174 GTGCAC
ApaLI 1886 GTGCAC G^TGCAC
2174 GTGCAC
BamHI 2134 GGATCC G^GATCC
5332 GGATCC
BglI 132 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4057 GCCAAACAGGC
BlpI 1377 GCTTAGC GC^T[ACGT]AGC
3691 GCTGAGC
Bpu1102I 1377 GCTTAGC GC^T[ACGT]AGC
3691 GCTGAGC
BsmI 739 GAATGC GAATGC(1/-1)
4107 GAATGC
BsrDI 982 GCAATG GCAATG(2/0)
1716 GCAATG
CelII 1377 GCTTAGC GC^T[ACGT]AGC
3691 GCTGAGC
DrdI 2094 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5126 GACTCTCCTGTC
HgaI 73 GACGC GACGC(5/10)
3904 GCGTC
MaeII 1478 ACGT A^CGT
1884 ACGT
SacI 4127 GAGCTC GAGCT^C
4517 GAGCTC
SapI 2069 GAAGAGC GCTCTTC(1/4)
3547 GAAGAGC
SmaI 125 CCCGGG CCC^GGG
181 CCCGGG
SspI 1738 AATATT AAT^ATT
5038 AATATT
XbaI 824 TCTAGA T^CTAGA
2453 TCTAGA
XmaCI 125 CCCGGG C^CCGGG
181 CCCGGG
XmaI 125 CCCGGG C^CCGGG
181 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI