##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI ApoI Asp700I Asp718I
AviII BciVI BfrI BsaBI BsiWI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EcoRI EcoRV EcoT22I FspI HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfuI SgrAI SnaBI SpeI
SphI SwaI Tsp509I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 4729 TCCGGA T^CCGGA
AhdI 3545 GACTGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1422 AGCGCT AGC^GCT
AscI 26 GGCGCGCC GG^CGCGCC
AseI 5222 ATTAAT AT^TAAT
AsnI 5222 ATTAAT AT^TAAT
AspEI 3545 GACTGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2879 GGATCC G^GATCC
BbeI 1446 GGCGCC GGCGC^C
BbsI 5187 GAAGAC GAAGAC(2/6)
BcgI 4100 GCAGTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2512 TGATCA T^GATCA
BglII 4651 AGATCT A^GATCT
BpuAI 5187 GAAGAC GAAGAC(2/6)
BseAI 4729 TCCGGA T^CCGGA
BsmI 4814 GCATTC GAATGC(1/-1)
Bsp1407I 3944 TGTACA T^GTACA
BspEI 4729 TCCGGA T^CCGGA
BsrGI 3944 TGTACA T^GTACA
BssHII 27 GCGCGC G^CGCGC
DraIII 2168 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3545 GACTGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1422 AGCGCT AGC^GCT
FbaI 2512 TGATCA T^GATCA
FseI 5091 GGCCGGCC GGCCGG^CC
HincII 792 GTTGAC GT[CT]^[GA]AC
HindII 792 GTTGAC GT[CT]^[GA]AC
KasI 1446 GGCGCC G^GCGCC
KspI 3816 CCGCGG CCGC^GG
MroI 4729 TCCGGA T^CCGGA
NarI 1446 GGCGCC GG^CGCC
NheI 2063 GCTAGC G^CTAGC
NspI 4859 GCATGT [GA]CATG^[CT]
PshBI 5222 ATTAAT AT^TAAT
SacII 3816 CCGCGG CCGC^GG
SapI 2389 GCTCTTC GCTCTTC(1/4)
SexAI 626 ACCTGGT A^CC[AT]GGT
SfoI 1446 GGCGCC GGC^GCC
SspBI 3944 TGTACA T^GTACA
SspI 208 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcyI 1446 GGCGCC G[GA]^CG[CT]C
3802 GACGCC
AflIII 332 ACGTGT A^C[GA][CT]GT
2437 ACGTGT
Alw44I 622 GTGCAC G^TGCAC
1098 GTGCAC
ApaLI 622 GTGCAC G^TGCAC
1098 GTGCAC
AvrII 2377 CCTAGG C^CTAGG
4610 CCTAGG
BlnI 2377 CCTAGG C^CTAGG
4610 CCTAGG
BsaHI 1446 GGCGCC G[GA]^CG[CT]C
3802 GACGCC
Hin1I 1446 GGCGCC G[GA]^CG[CT]C
3802 GACGCC
PshAI 3674 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3926 GACAGGAGTC
SfiI 714 GGCCTACAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1776 GGCCCCGGGGGCC
SmlI 196 CTCAAG C^T[CT][GA]AG
1405 CTCAAG
StuI 3466 AGGCCT AGG^CCT
4613 AGGCCT
XcmI 371 CCATTGGGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4695 CCAGTAGGGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
Sse8387I StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI