##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI Aor51HI AscI Asp718I
BamHI BbeI BclI BsaBI BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII Eco47III EcoO65I EcoRI EcoT22I
FbaI FseI HincII HindII HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
Psp1406I PvuI RsrII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3143 GACGTC GACGT^C
AccI 3407 GTCTAC GT^[AC][GT]AC
AflII 2065 CTTAAG C^TTAAG
AgeI 1876 ACCGGT A^CCGGT
AhdI 1167 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4131 GTGCAC G^TGCAC
ApaLI 4131 GTGCAC G^TGCAC
AseI 2612 ATTAAT AT^TAAT
AsnI 2612 ATTAAT AT^TAAT
Asp700I 1496 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1167 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 2065 CTTAAG C^TTAAG
Bsp1407I 2145 TGTACA T^GTACA
BspLU11I 4579 ACATGT A^CATGT
BspMI 3821 GCAGGT ACCTGC(4/8)
BsrDI 4348 GCAATG GCAATG(2/0)
BsrGI 2145 TGTACA T^GTACA
BssSI 1350 CTCGTG CACGAG(-5/-1)
Eam1105I 1167 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3342 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 4823 AAGCTT A^AGCTT
KspI 772 CCGCGG CCGC^GG
NaeI 559 GCCGGC GCC^GGC
NgoMIV 559 GCCGGC G^CCGGC
PinAI 1876 ACCGGT A^CCGGT
PshBI 2612 ATTAAT AT^TAAT
SacI 4183 GAGCTC GAGCT^C
SacII 772 CCGCGG CCGC^GG
SapI 495 GAAGAGC GCTCTTC(1/4)
SbfI 617 CCTGCAGG CCTGCA^GG
ScaI 2099 AGTACT AGT^ACT
SphI 599 GCATGC GCATG^C
Sse8387I 617 CCTGCAGG CCTGCA^GG
SspBI 2145 TGTACA T^GTACA
StuI 3015 AGGCCT AGG^CCT
XmnI 1496 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 3358 TCCGGA T^CCGGA
4907 TCCGGA
AflIII 3837 ACACGT A^C[GA][CT]GT
4579 ACATGT
AviII 1111 TGCGCA TGC^GCA
1213 TGCGCA
BalI 1399 TGGCCA TGG^CCA
3751 TGGCCA
BbrPI 1978 CACGTG CAC^GTG
3838 CACGTG
BglII 181 AGATCT A^GATCT
1367 AGATCT
BlpI 1577 GCTGAGC GC^T[ACGT]AGC
3270 GCTGAGC
Bpu1102I 1577 GCTGAGC GC^T[ACGT]AGC
3270 GCTGAGC
BsaAI 1978 CACGTG [CT]AC^GT[GA]
3838 CACGTG
BseAI 3358 TCCGGA T^CCGGA
4907 TCCGGA
BspEI 3358 TCCGGA T^CCGGA
4907 TCCGGA
BstAPI 594 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1100 GCAGACCCTGC
CelII 1577 GCTGAGC GC^T[ACGT]AGC
3270 GCTGAGC
DrdI 2208 GACGGTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2408 GACGCTGGCGTC
EagI 335 CGGCCG C^GGCCG
4253 CGGCCG
EarI 25 GAAGAG CTCTTC(1/4)
495 GAAGAG
EclXI 335 CGGCCG C^GGCCG
4253 CGGCCG
Eco52I 335 CGGCCG C^GGCCG
4253 CGGCCG
FspI 1111 TGCGCA TGC^GCA
1213 TGCGCA
HaeII 1256 GGCGCT [GA]GCGC^[CT]
1300 GGCGCT
Ksp632I 25 GAAGAG CTCTTC(1/4)
495 GAAGAG
MluNI 1399 TGGCCA TGG^CCA
3751 TGGCCA
MroI 3358 TCCGGA T^CCGGA
4907 TCCGGA
MscI 1399 TGGCCA TGG^CCA
3751 TGGCCA
NcoI 3059 CCATGG C^CATGG
3234 CCATGG
PmaCI 1978 CACGTG CAC^GTG
3838 CACGTG
PmlI 1978 CACGTG CAC^GTG
3838 CACGTG
PshAI 1422 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2374 GACCACGGTC
SspI 2576 AATATT AAT^ATT
2918 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
AvrII BanI BanII BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI