##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AclI AcsI AgeI
Alw44I ApaLI ApoI AscI AseI
AsnI AspI AvrII BamHI BcgI
BciVI BclI BlnI BsaBI BsiEI
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI HindIII HpaI KspI
MamI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfuI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 798 GGCGCC G[GA]^CG[CT]C
AflII 1457 CTTAAG C^TTAAG
AflIII 3346 ACATGT A^C[GA][CT]GT
AhdI 3342 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1360 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3342 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3883 TGCGCA TGC^GCA
BbeI 798 GGCGCC GGCGC^C
BfrI 1457 CTTAAG C^TTAAG
BglII 4521 AGATCT A^GATCT
BsaHI 798 GGCGCC G[GA]^CG[CT]C
BsmBI 3822 GAGACG CGTCTC(1/5)
Bsp1407I 4927 TGTACA T^GTACA
BspHI 4030 TCATGA T^CATGA
BspLU11I 3346 ACATGT A^CATGT
BsrGI 4927 TGTACA T^GTACA
BssSI 2041 CACGAG CACGAG(-5/-1)
BstEII 4582 GGTTACC G^GT[ACGT]ACC
BstPI 4582 GGTTACC G^GT[ACGT]ACC
Eam1105I 3342 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4582 GGTTACC G^GT[ACGT]ACC
FseI 2555 GGCCGGCC GGCCGG^CC
FspI 3883 TGCGCA TGC^GCA
Hin1I 798 GGCGCC G[GA]^CG[CT]C
KasI 798 GGCGCC G^GCGCC
NarI 798 GGCGCC GG^CGCC
NheI 2219 GCTAGC G^CTAGC
PshAI 24 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4030 TCATGA T^CATGA
SexAI 202 ACCTGGT A^CC[AT]GGT
SfiI 1419 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 798 GGCGCC GGC^GCC
SgrAI 1857 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2689 CCCGGG CCC^GGG
SspBI 4927 TGTACA T^GTACA
SspI 4095 AATATT AAT^ATT
XmaCI 2689 CCCGGG C^CCGGG
XmaI 2689 CCCGGG C^CCGGG
XmnI 1360 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 932 GGTACC G^GTACC
4335 GGTACC
AccIII 2572 TCCGGA T^CCGGA
3622 TCCGGA
AocI 71 CCTTAGG CC^T[ACGT]AGG
1971 CCTGAGG
Asp718I 932 GGTACC G^GTACC
4335 GGTACC
BalI 1156 TGGCCA TGG^CCA
2623 TGGCCA
BbrPI 2256 CACGTG CAC^GTG
3690 CACGTG
BsaWI 2572 TCCGGA [AT]^CCGG[AT]
3622 TCCGGA
BseAI 2572 TCCGGA T^CCGGA
3622 TCCGGA
BsmI 2699 GAATGC GAATGC(1/-1)
1210 GCATTC
BspEI 2572 TCCGGA T^CCGGA
3622 TCCGGA
BsrDI 4602 CATTGC GCAATG(2/0)
379 GCAATG
Bsu36I 71 CCTTAGG CC^T[ACGT]AGG
1971 CCTGAGG
Eco81I 71 CCTTAGG CC^T[ACGT]AGG
1971 CCTGAGG
HgaI 84 GACGC GACGC(5/10)
1601 GACGC
HincII 1198 GTCAAC GT[CT]^[GA]AC
4532 GTCAAC
HindII 1198 GTCAAC GT[CT]^[GA]AC
4532 GTCAAC
KpnI 932 GGTACC GGTAC^C
4335 GGTACC
MluNI 1156 TGGCCA TGG^CCA
2623 TGGCCA
MroI 2572 TCCGGA T^CCGGA
3622 TCCGGA
MscI 1156 TGGCCA TGG^CCA
2623 TGGCCA
NaeI 1420 GCCGGC GCC^GGC
2556 GCCGGC
NgoMIV 1420 GCCGGC G^CCGGC
2556 GCCGGC
NspI 439 GCATGT [GA]CATG^[CT]
3346 ACATGT
PmaCI 2256 CACGTG CAC^GTG
3690 CACGTG
PmlI 2256 CACGTG CAC^GTG
3690 CACGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII