##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AclI AflII Aor51HI AscI
BbeI BciVI BfrI BsiEI BsiWI
BsmBI Bsp1407I BspLU11I BsrGI BssHII
BstBI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HpaI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PmeI Psp1406I PvuI
RsrII SacII SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3458 GGTACC G^GTACC
AccII 189 CGCG CG^CG
AccIII 725 TCCGGA T^CCGGA
AcyI 1901 GGCGTC G[GA]^CG[CT]C
AflIII 707 ACGTGT A^C[GA][CT]GT
AgeI 576 ACCGGT A^CCGGT
AhdI 1348 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2880 GTGCAC G^TGCAC
ApaLI 2880 GTGCAC G^TGCAC
AseI 5470 ATTAAT AT^TAAT
AsnI 5470 ATTAAT AT^TAAT
Asp700I 2463 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3458 GGTACC G^GTACC
AspEI 1348 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4585 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 905 TGCGCA TGC^GCA
BamHI 662 GGATCC G^GATCC
BglII 4555 AGATCT A^GATCT
BsaBI 4783 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1901 GGCGTC G[GA]^CG[CT]C
BseAI 725 TCCGGA T^CCGGA
BsmI 700 GCATTC GAATGC(1/-1)
BspDI 562 ATCGAT AT^CGAT
BspEI 725 TCCGGA T^CCGGA
Bst1107I 762 GTATAC GTA^TAC
BstEII 1111 GGTGACC G^GT[ACGT]ACC
BstPI 1111 GGTGACC G^GT[ACGT]ACC
BstUI 189 CGCG CG^CG
BstZ17I 762 GTATAC GTA^TAC
ClaI 562 ATCGAT AT^CGAT
DraI 4692 TTTAAA TTT^AAA
Eam1105I 1348 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1111 GGTGACC G^GT[ACGT]ACC
FspI 905 TGCGCA TGC^GCA
Hin1I 1901 GGCGTC G[GA]^CG[CT]C
HindIII 4187 AAGCTT A^AGCTT
KpnI 3458 GGTACC GGTAC^C
MamI 4783 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 725 TCCGGA T^CCGGA
MvnI 189 CGCG CG^CG
NaeI 1031 GCCGGC GCC^GGC
NgoMIV 1031 GCCGGC G^CCGGC
PaeR7I 2527 CTCGAG C^TCGAG
PflFI 4585 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 576 ACCGGT A^CCGGT
PshAI 1949 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5470 ATTAAT AT^TAAT
SalI 997 GTCGAC G^TCGAC
ScaI 4768 AGTACT AGT^ACT
Tth111I 4585 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2527 CTCGAG C^TCGAG
XmnI 2463 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BalI 104 TGGCCA TGG^CCA
269 TGGCCA
BbvCI 1200 CCTCAGC CCTCAGC(-5/-2)
4367 GCTGAGG
BcgI 942 CGAGTTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5377 GCATTTCGCTCG
BlpI 2280 GCTCAGC GC^T[ACGT]AGC
3830 GCTAAGC
Bpu1102I 2280 GCTCAGC GC^T[ACGT]AGC
3830 GCTAAGC
BsaWI 576 ACCGGT [AT]^CCGG[AT]
725 TCCGGA
BsrBI 241 GAGCGG CCGCTC(-3/-3)
1107 GAGCGG
BssSI 3223 CACGAG CACGAG(-5/-1)
535 CTCGTG
CelII 2280 GCTCAGC GC^T[ACGT]AGC
3830 GCTAAGC
DraIII 1221 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4724 CACCAGGTG
DrdI 1209 GACAACCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3483 GACCGTAGAGTC
EaeI 104 TGGCCA [CT]^GGCC[GA]
269 TGGCCA
EcoT22I 472 ATGCAT ATGCA^T
698 ATGCAT
HincII 997 GTCGAC GT[CT]^[GA]AC
3795 GTCAAC
HindII 997 GTCGAC GT[CT]^[GA]AC
3795 GTCAAC
MluNI 104 TGGCCA TGG^CCA
269 TGGCCA
MscI 104 TGGCCA TGG^CCA
269 TGGCCA
NsiI 472 ATGCAT ATGCA^T
698 ATGCAT
NspI 2737 GCATGT [GA]CATG^[CT]
3410 GCATGT
PstI 1968 CTGCAG CTGCA^G
5149 CTGCAG
SacI 556 GAGCTC GAGCT^C
2235 GAGCTC
SfiI 96 GGCCACTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1822 GGCCACCTAGGCC
SmaI 1067 CCCGGG CCC^GGG
1079 CCCGGG
SspI 748 AATATT AAT^ATT
5460 AATATT
StuI 3737 AGGCCT AGG^CCT
4980 AGGCCT
XmaCI 1067 CCCGGG C^CCGGG
1079 CCCGGG
XmaI 1067 CCCGGG C^CCGGG
1079 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AvrII BanI
BanII BbrPI BbsI BbvI BclI
BcnI BfaI BglI BlnI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PpuMI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII