##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AgeI Aor51HI AscI
AseI AsnI AviII BfrI BspDI
BspLU11I BssHII BssSI BstBI ClaI
CpoI Eco47III EcoRV FseI FspI
KspI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SfiI SfuI SgrAI SmlI SnaBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1263 GACGTC GACGT^C
Acc65I 1932 GGTACC G^GTACC
AccII 2612 CGCG CG^CG
AccIII 33 TCCGGA T^CCGGA
AflIII 5140 ACGTGT A^C[GA][CT]GT
AhdI 5001 GACGCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1399 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1932 GGTACC G^GTACC
AspEI 5001 GACGCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsaBI 2489 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 33 TCCGGA T^CCGGA
BsmBI 214 GAGACG CGTCTC(1/5)
BsmI 1040 GCATTC GAATGC(1/-1)
BspEI 33 TCCGGA T^CCGGA
BsrDI 3819 CATTGC GCAATG(2/0)
BsrFI 3823 GCCGGC [GA]^CCGG[CT]
BstUI 2612 CGCG CG^CG
Cfr10I 3823 GCCGGC [GA]^CCGG[CT]
EagI 4339 CGGCCG C^GGCCG
Eam1105I 5001 GACGCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4339 CGGCCG C^GGCCG
Eco52I 4339 CGGCCG C^GGCCG
EcoRI 4717 GAATTC G^AATTC
EcoT22I 1211 ATGCAT ATGCA^T
HpaI 1339 GTTAAC GTT^AAC
KpnI 1932 GGTACC GGTAC^C
MamI 2489 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 33 TCCGGA T^CCGGA
MvnI 2612 CGCG CG^CG
NaeI 3823 GCCGGC GCC^GGC
NgoMIV 3823 GCCGGC G^CCGGC
NsiI 1211 ATGCAT ATGCA^T
SalI 388 GTCGAC G^TCGAC
SapI 3789 GCTCTTC GCTCTTC(1/4)
SbfI 3561 CCTGCAGG CCTGCA^GG
ScaI 1204 AGTACT AGT^ACT
SpeI 1022 ACTAGT A^CTAGT
SphI 3234 GCATGC GCATG^C
Sse8387I 3561 CCTGCAGG CCTGCA^GG
SspI 4673 AATATT AAT^ATT
XcmI 2295 CCACACAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1399 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 55 GTGCAC G^TGCAC
4038 GTGCAC
ApaLI 55 GTGCAC G^TGCAC
4038 GTGCAC
AspI 382 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTGGTC
AvrII 3924 CCTAGG C^CTAGG
5112 CCTAGG
BamHI 3668 GGATCC G^GATCC
3928 GGATCC
BbeI 67 GGCGCC GGCGC^C
2911 GGCGCC
BcgI 263 GCAAAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2682 GCAGCAGGGTCG
BclI 1293 TGATCA T^GATCA
1359 TGATCA
BlnI 3924 CCTAGG C^CTAGG
5112 CCTAGG
BlpI 2248 GCTTAGC GC^T[ACGT]AGC
4250 GCTCAGC
Bpu1102I 2248 GCTTAGC GC^T[ACGT]AGC
4250 GCTCAGC
BsaWI 33 TCCGGA [AT]^CCGG[AT]
1170 TCCGGT
BsiEI 273 CGACCG CG[GA][CT]^CG
4339 CGGCCG
BsiWI 692 CGTACG C^GTACG
3356 CGTACG
Bsp1407I 954 TGTACA T^GTACA
5045 TGTACA
BspHI 1109 TCATGA T^CATGA
4120 TCATGA
BsrGI 954 TGTACA T^GTACA
5045 TGTACA
Bst1107I 946 GTATAC GTA^TAC
5576 GTATAC
BstAPI 3229 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4031 GCAGCATGTGC
BstEII 595 GGTGACC G^GT[ACGT]ACC
4368 GGTCACC
BstPI 595 GGTGACC G^GT[ACGT]ACC
4368 GGTCACC
BstZ17I 946 GTATAC GTA^TAC
5576 GTATAC
CelII 2248 GCTTAGC GC^T[ACGT]AGC
4250 GCTCAGC
DraIII 22 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2741 CACAGAGTG
DrdI 502 GACTTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
598 GACCAGCTAGTC
EcoNI 3420 CCTATAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3561 CCTGCAGGAGG
EcoO65I 595 GGTGACC G^GT[ACGT]ACC
4368 GGTCACC
FbaI 1293 TGATCA T^GATCA
1359 TGATCA
HaeII 67 GGCGCC [GA]GCGC^[CT]
2911 GGCGCC
HgaI 5001 GACGC GACGC(5/10)
1029 GCGTC
KasI 67 GGCGCC G^GCGCC
2911 GGCGCC
NarI 67 GGCGCC GG^CGCC
2911 GGCGCC
NcoI 305 CCATGG C^CATGG
2165 CCATGG
PflFI 382 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTGGTC
RcaI 1109 TCATGA T^CATGA
4120 TCATGA
SexAI 3710 ACCTGGT A^CC[AT]GGT
4042 ACCAGGT
SfoI 67 GGCGCC GGC^GCC
2911 GGCGCC
SmaI 1588 CCCGGG CCC^GGG
3000 CCCGGG
SspBI 954 TGTACA T^GTACA
5045 TGTACA
Tth111I 382 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3709 GACCTGGTC
XmaCI 1588 CCCGGG C^CCGGG
3000 CCCGGG
XmaI 1588 CCCGGG C^CCGGG
3000 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII