##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BciVI BclI BcnI
BglII BlnI BsaAI BsaHI BsiEI
BsiWI BspHI BsrBI BsrFI BssHII
BstBI BstEII BstPI Cfr10I CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I FbaI FseI FspI
HgaI Hin1I KasI KspI MfeI
MluI MunI NaeI NarI NciI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1554 GGTACC G^GTACC
AccI 3531 GTATAC GT^[AC][GT]AC
AccII 4133 CGCG CG^CG
AccIII 3944 TCCGGA T^CCGGA
Alw44I 1323 GTGCAC G^TGCAC
Aor51HI 1353 AGCGCT AGC^GCT
ApaI 3577 GGGCCC GGGCC^C
ApaLI 1323 GTGCAC G^TGCAC
Asp718I 1554 GGTACC G^GTACC
BbvCI 1814 CCTCAGC CCTCAGC(-5/-2)
BcgI 3126 CGATTATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 1998 GAGACC GGTCTC(1/5)
BseAI 3944 TCCGGA T^CCGGA
BsmBI 3872 CGTCTC CGTCTC(1/5)
BsmI 4578 GCATTC GAATGC(1/-1)
Bsp1407I 4918 TGTACA T^GTACA
BspEI 3944 TCCGGA T^CCGGA
BspMI 1600 ACCTGC ACCTGC(4/8)
BsrGI 4918 TGTACA T^GTACA
BssSI 4891 CTCGTG CACGAG(-5/-1)
Bst1107I 3531 GTATAC GTA^TAC
BstUI 4133 CGCG CG^CG
BstXI 4736 CCAACTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3531 GTATAC GTA^TAC
DraIII 1137 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 704 GACGTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1353 AGCGCT AGC^GCT
EcoT22I 2550 ATGCAT ATGCA^T
FauI 3938 CCCGC CCCGC(4/6)
HpaI 5244 GTTAAC GTT^AAC
KpnI 1554 GGTACC GGTAC^C
MroI 3944 TCCGGA T^CCGGA
MvnI 4133 CGCG CG^CG
NsiI 2550 ATGCAT ATGCA^T
PaeR7I 4470 CTCGAG C^TCGAG
ScaI 5047 AGTACT AGT^ACT
SexAI 4439 ACCTGGT A^CC[AT]GGT
SspBI 4918 TGTACA T^GTACA
StuI 1460 AGGCCT AGG^CCT
XbaI 4463 TCTAGA T^CTAGA
XhoI 4470 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 62 CTTAAG C^TTAAG
4701 CTTAAG
AocI 1059 CCTGAGG CC^T[ACGT]AGG
3903 CCTCAGG
AseI 2190 ATTAAT AT^TAAT
3642 ATTAAT
AsnI 2190 ATTAAT AT^TAAT
3642 ATTAAT
BfrI 62 CTTAAG C^TTAAG
4701 CTTAAG
BglI 1423 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2562 GCCAAAGTGGC
BpmI 759 CTGGAG CTGGAG(16/14)
2695 CTGGAG
BsaBI 1164 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5297 GATGGATATC
BsaWI 53 TCCGGT [AT]^CCGG[AT]
3944 TCCGGA
BspDI 2873 ATCGAT AT^CGAT
3172 ATCGAT
BspLU11I 225 ACATGT A^CATGT
3736 ACATGT
BstAPI 1304 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2543 GCAGACGATGC
Bsu36I 1059 CCTGAGG CC^T[ACGT]AGG
3903 CCTCAGG
ClaI 2873 ATCGAT AT^CGAT
3172 ATCGAT
Eco81I 1059 CCTGAGG CC^T[ACGT]AGG
3903 CCTCAGG
HapII 54 CCGG C^CGG
3945 CCGG
HpaII 54 CCGG C^CGG
3945 CCGG
MamI 1164 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5297 GATGGATATC
MspI 54 CCGG C^CGG
3945 CCGG
NcoI 1662 CCATGG C^CATGG
4616 CCATGG
NheI 2288 GCTAGC G^CTAGC
3819 GCTAGC
PshBI 2190 ATTAAT AT^TAAT
3642 ATTAAT
PvuII 1474 CAGCTG CAG^CTG
3951 CAGCTG
SacI 725 GAGCTC GAGCT^C
2245 GAGCTC
SapI 3963 GAAGAGC GCTCTTC(1/4)
2398 GCTCTTC
SfcI 1159 CTATAG C^T[GA][CT]AG
2203 CTGTAG
SpeI 72 ACTAGT A^CTAGT
4935 ACTAGT
SphI 2162 GCATGC GCATG^C
4782 GCATGC
SspI 2768 AATATT AAT^ATT
5356 AATATT
XcmI 1779 CCAACTGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3814 CCAAAGCTAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BfaI BlpI
BmyI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoRV EcoT14I Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI