##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
Acc65I AflII AgeI AhdI AscI
Asp718I AspEI AviII BciVI BclI
BfrI BsiWI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I EcoRI
EcoRV FbaI FspI HindIII KpnI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SpeI SphI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3999 GACGTC GACGT^C
AccI 1659 GTCTAC GT^[AC][GT]AC
AccIII 1764 TCCGGA T^CCGGA
AseI 2667 ATTAAT AT^TAAT
AsnI 2667 ATTAAT AT^TAAT
Asp700I 2901 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 4354 CCTAGG C^CTAGG
BbeI 1398 GGCGCC GGCGC^C
BbrPI 239 CACGTG CAC^GTG
BlnI 4354 CCTAGG C^CTAGG
BsaWI 1764 TCCGGA [AT]^CCGG[AT]
BseAI 1764 TCCGGA T^CCGGA
BsiEI 2115 CGGCCG CG[GA][CT]^CG
BspEI 1764 TCCGGA T^CCGGA
BspLU11I 922 ACATGT A^CATGT
BstAPI 194 GCAGAACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 3188 GACCACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2115 CGGCCG C^GGCCG
EclXI 2115 CGGCCG C^GGCCG
Eco52I 2115 CGGCCG C^GGCCG
EcoT22I 1444 ATGCAT ATGCA^T
FseI 2116 GGCCGGCC GGCCGG^CC
HpaI 2576 GTTAAC GTT^AAC
KasI 1398 GGCGCC G^GCGCC
MroI 1764 TCCGGA T^CCGGA
NarI 1398 GGCGCC GG^CGCC
NsiI 1444 ATGCAT ATGCA^T
PflMI 4712 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 239 CACGTG CAC^GTG
PmlI 239 CACGTG CAC^GTG
PshBI 2667 ATTAAT AT^TAAT
SapI 2209 GAAGAGC GCTCTTC(1/4)
SbfI 572 CCTGCAGG CCTGCA^GG
SfoI 1398 GGCGCC GGC^GCC
SnaBI 2557 TACGTA TAC^GTA
Sse8387I 572 CCTGCAGG CCTGCA^GG
SspI 3715 AATATT AAT^ATT
Van91I 4712 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2901 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 2944 AAATTT [GA]^AATT[CT]
3880 GAATTT
Aor51HI 661 AGCGCT AGC^GCT
3920 AGCGCT
ApoI 2944 AAATTT [GA]^AATT[CT]
3880 GAATTT
BamHI 780 GGATCC G^GATCC
5091 GGATCC
BbvCI 965 CCTCAGC CCTCAGC(-5/-2)
3067 CCTCAGC
BglI 480 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1348 GCCCCGGCGGC
BglII 2098 AGATCT A^GATCT
5243 AGATCT
BsaAI 239 CACGTG [CT]AC^GT[GA]
2557 TACGTA
BsaBI 1136 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4373 GATTTTAATC
BsaI 3425 GAGACC GGTCTC(1/5)
3908 GAGACC
BsmI 2634 GAATGC GAATGC(1/-1)
4998 GCATTC
BsrDI 1979 GCAATG GCAATG(2/0)
4286 GCAATG
BssSI 2520 CACGAG CACGAG(-5/-1)
2761 CTCGTG
DraIII 4571 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
5008 CACACAGTG
Eco47III 661 AGCGCT AGC^GCT
3920 AGCGCT
HincII 2505 GTCAAC GT[CT]^[GA]AC
2576 GTTAAC
HindII 2505 GTCAAC GT[CT]^[GA]AC
2576 GTTAAC
KspI 942 CCGCGG CCGC^GG
2512 CCGCGG
MamI 1136 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4373 GATTTTAATC
NaeI 2117 GCCGGC GCC^GGC
4156 GCCGGC
NgoMIV 2117 GCCGGC G^CCGGC
4156 GCCGGC
PvuII 228 CAGCTG CAG^CTG
1620 CAGCTG
SacII 942 CCGCGG CCGC^GG
2512 CCGCGG
SexAI 400 ACCTGGT A^CC[AT]GGT
1326 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AciI AclI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI Psp1406I PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmaCI XmaI