##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AclI AflII AflIII AgeI AscI
BbeI BfrI BsiWI BspLU11I Bst1107I
BstEII BstPI BstZ17I CpoI EcoO65I
FseI KasI KspI MfeI MluI
MunI NarI NcoI NdeI NotI
NruI PacI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 5211 GACGTC GACGT^C
AccI 592 GTCTAC GT^[AC][GT]AC
AcyI 5211 GACGTC G[GA]^CG[CT]C
AseI 1242 ATTAAT AT^TAAT
AsnI 1242 ATTAAT AT^TAAT
AviII 2644 TGCGCA TGC^GCA
AvrII 4821 CCTAGG C^CTAGG
BamHI 3887 GGATCC G^GATCC
BcgI 2434 GCACTGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 760 AGATCT A^GATCT
BlnI 4821 CCTAGG C^CTAGG
BsaBI 3888 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5211 GACGTC G[GA]^CG[CT]C
BsaI 580 GAGACC GGTCTC(1/5)
Bsp1407I 1562 TGTACA T^GTACA
BspDI 561 ATCGAT AT^CGAT
BspHI 5598 TCATGA T^CATGA
BsrGI 1562 TGTACA T^GTACA
BssHII 5426 GCGCGC G^CGCGC
BstBI 2878 TTCGAA TT^CGAA
ClaI 561 ATCGAT AT^CGAT
DraI 1634 TTTAAA TTT^AAA
EcoRV 1574 GATATC GAT^ATC
FspI 2644 TGCGCA TGC^GCA
Hin1I 5211 GACGTC G[GA]^CG[CT]C
MamI 3888 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5255 GCCGGC GCC^GGC
NgoMIV 5255 GCCGGC G^CCGGC
NheI 4087 GCTAGC G^CTAGC
NspV 2878 TTCGAA TT^CGAA
PaeR7I 1705 CTCGAG C^TCGAG
PflMI 518 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1242 ATTAAT AT^TAAT
RcaI 5598 TCATGA T^CATGA
ScaI 1982 AGTACT AGT^ACT
SfuI 2878 TTCGAA TT^CGAA
SspBI 1562 TGTACA T^GTACA
Van91I 518 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4107 TCTAGA T^CTAGA
XhoI 1705 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2992 GGTACC G^GTACC
4784 GGTACC
AccIII 4707 TCCGGA T^CCGGA
5440 TCCGGA
Aor51HI 2931 AGCGCT AGC^GCT
5139 AGCGCT
ApaI 4976 GGGCCC GGGCC^C
5511 GGGCCC
Asp718I 2992 GGTACC G^GTACC
4784 GGTACC
BbrPI 4319 CACGTG CAC^GTG
4558 CACGTG
BciVI 870 GTATCC GTATCC(6/5)
4721 GTATCC
BclI 3017 TGATCA T^GATCA
4628 TGATCA
BglI 4490 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5530 GCCAGCCAGGC
BseAI 4707 TCCGGA T^CCGGA
5440 TCCGGA
BsiEI 736 CGGCCG CG[GA][CT]^CG
5730 CGGCCG
BsmBI 82 CGTCTC CGTCTC(1/5)
3935 CGTCTC
BsmI 2943 GAATGC GAATGC(1/-1)
4645 GAATGC
BspEI 4707 TCCGGA T^CCGGA
5440 TCCGGA
BsrBI 4238 CCGCTC CCGCTC(-3/-3)
5338 GAGCGG
DraIII 4558 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
5062 CACGGGGTG
DrdI 1770 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4435 GACCCAGCAGTC
EagI 736 CGGCCG C^GGCCG
5730 CGGCCG
EclXI 736 CGGCCG C^GGCCG
5730 CGGCCG
Eco47III 2931 AGCGCT AGC^GCT
5139 AGCGCT
Eco52I 736 CGGCCG C^GGCCG
5730 CGGCCG
EcoRI 323 GAATTC G^AATTC
3649 GAATTC
EcoT22I 1590 ATGCAT ATGCA^T
2960 ATGCAT
FbaI 3017 TGATCA T^GATCA
4628 TGATCA
HindIII 71 AAGCTT A^AGCTT
4608 AAGCTT
HpaI 1131 GTTAAC GTT^AAC
4636 GTTAAC
KpnI 2992 GGTACC GGTAC^C
4784 GGTACC
MroI 4707 TCCGGA T^CCGGA
5440 TCCGGA
NsiI 1590 ATGCAT ATGCA^T
2960 ATGCAT
PmaCI 4319 CACGTG CAC^GTG
4558 CACGTG
PmlI 4319 CACGTG CAC^GTG
4558 CACGTG
SacI 4223 GAGCTC GAGCT^C
5166 GAGCTC
SexAI 53 ACCTGGT A^CC[AT]GGT
4885 ACCTGGT
SmaI 358 CCCGGG CCC^GGG
1069 CCCGGG
XcmI 643 CCATGACAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3404 CCAACACAAGACTGG
XmaCI 358 CCCGGG C^CCGGG
1069 CCCGGG
XmaI 358 CCCGGG C^CCGGG
1069 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspEI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PshAI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI