##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BbrPI BcgI BglI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrFI BssHII BstAPI
BstBI BstUI BstXI Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I FseI FspI KpnI KspI
MluI MroI MvnI NaeI NgoMIV
NotI NruI NspV PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2160 GTATAC GT^[AC][GT]AC
AcyI 4763 GGCGCC G[GA]^CG[CT]C
AhdI 3934 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2288 GGGCCC GGGCC^C
AspEI 3934 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 4763 GGCGCC G^G[CT][GA]CC
BbeI 4763 GGCGCC GGCGC^C
BclI 2886 TGATCA T^GATCA
BsaBI 5795 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4763 GGCGCC G[GA]^CG[CT]C
BsaI 401 GAGACC GGTCTC(1/5)
BsgI 336 GTGCAG GTGCAG(16/14)
Bsp1407I 5556 TGTACA T^GTACA
BspHI 2643 TCATGA T^CATGA
BsrGI 5556 TGTACA T^GTACA
BssSI 854 CTCGTG CACGAG(-5/-1)
Bst1107I 2160 GTATAC GTA^TAC
BstEII 5265 GGTCACC G^GT[ACGT]ACC
BstPI 5265 GGTCACC G^GT[ACGT]ACC
BstZ17I 2160 GTATAC GTA^TAC
CfoI 4764 GCGC GCG^C
DrdI 3717 GACTGCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3934 GACAAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5265 GGTCACC G^GT[ACGT]ACC
EcoRI 1259 GAATTC G^AATTC
EcoRV 3639 GATATC GAT^ATC
EcoT22I 494 ATGCAT ATGCA^T
FbaI 2886 TGATCA T^GATCA
HaeII 4763 GGCGCC [GA]GCGC^[CT]
HhaI 4764 GCGC GCG^C
Hin1I 4763 GGCGCC G[GA]^CG[CT]C
HinP1I 4764 GCGC G^CGC
HpaI 2869 GTTAAC GTT^AAC
KasI 4763 GGCGCC G^GCGCC
MamI 5795 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4763 GGCGCC GG^CGCC
NcoI 422 CCATGG C^CATGG
NheI 1386 GCTAGC G^CTAGC
NsiI 494 ATGCAT ATGCA^T
PacI 5191 TTAATTAA TTAAT^TAA
PshAI 381 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2643 TCATGA T^CATGA
SbfI 3096 CCTGCAGG CCTGCA^GG
ScaI 1931 AGTACT AGT^ACT
SfoI 4763 GGCGCC GGC^GCC
SpeI 4230 ACTAGT A^CTAGT
Sse8387I 3096 CCTGCAGG CCTGCA^GG
SspBI 5556 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 5190 ATTAAT AT^TAAT
5528 ATTAAT
AsnI 5190 ATTAAT AT^TAAT
5528 ATTAAT
AvaI 1532 CCCGAG C^[CT]CG[GA]G
3728 CCCGAG
BalI 1370 TGGCCA TGG^CCA
4622 TGGCCA
BbvCI 5369 CCTCAGC CCTCAGC(-5/-2)
2833 GCTGAGG
BciVI 2014 GGATAC GTATCC(6/5)
2149 GGATAC
BcnI 5115 CCGGG CC^[GC]GG
5133 CCGGG
BsoBI 1532 CCCGAG C^[CT]CG[GA]G
3728 CCCGAG
BsrBI 93 GAGCGG CCGCTC(-3/-3)
4753 GAGCGG
DsaI 422 CCATGG C^C[GA][CT]GG
1367 CCGTGG
EaeI 1370 TGGCCA [CT]^GGCC[GA]
4622 TGGCCA
HgaI 5127 GCGTC GACGC(5/10)
5290 GCGTC
HindIII 2517 AAGCTT A^AGCTT
5007 AAGCTT
MfeI 4501 CAATTG C^AATTG
4724 CAATTG
MluNI 1370 TGGCCA TGG^CCA
4622 TGGCCA
MscI 1370 TGGCCA TGG^CCA
4622 TGGCCA
MunI 4501 CAATTG C^AATTG
4724 CAATTG
NciI 5115 CCGGG CC^[GC]GG
5133 CCGGG
PshBI 5190 ATTAAT AT^TAAT
5528 ATTAAT
SapI 3037 GAAGAGC GCTCTTC(1/4)
4402 GAAGAGC
SspI 2488 AATATT AAT^ATT
3502 AATATT
StuI 1085 AGGCCT AGG^CCT
1212 AGGCCT
XcmI 38 CCATAGATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
813 CCATGTGGTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanII BbsI
BbvI BfaI BfrI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII
XmnI