##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BalI BamHI BclI BfrI BlnI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SfuI SnaBI SpeI SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 471 GGTACC G^GTACC
AccIII 1967 TCCGGA T^CCGGA
AcsI 5554 AAATTT [GA]^AATT[CT]
Aor51HI 2100 AGCGCT AGC^GCT
ApoI 5554 AAATTT [GA]^AATT[CT]
Asp718I 471 GGTACC G^GTACC
BbeI 4458 GGCGCC GGCGC^C
BsaWI 1967 TCCGGA [AT]^CCGG[AT]
BseAI 1967 TCCGGA T^CCGGA
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsmBI 935 GAGACG CGTCTC(1/5)
BspEI 1967 TCCGGA T^CCGGA
BssSI 4816 CACGAG CACGAG(-5/-1)
EagI 104 CGGCCG C^GGCCG
EclXI 104 CGGCCG C^GGCCG
Eco47III 2100 AGCGCT AGC^GCT
Eco52I 104 CGGCCG C^GGCCG
KasI 4458 GGCGCC G^GCGCC
KpnI 471 GGTACC GGTAC^C
MroI 1967 TCCGGA T^CCGGA
NarI 4458 GGCGCC GG^CGCC
NruI 2126 TCGCGA TCG^CGA
ScaI 3571 AGTACT AGT^ACT
SexAI 5093 ACCAGGT A^CC[AT]GGT
SfoI 4458 GGCGCC GGC^GCC
SgrAI 163 CGCCGGTG C[GA]^CCGG[CT]G
XbaI 3864 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 173 GACGTC GACGT^C
3018 GACGTC
AccI 3135 GTAGAC GT^[AC][GT]AC
4612 GTAGAC
Alw44I 2555 GTGCAC G^TGCAC
2788 GTGCAC
ApaLI 2555 GTGCAC G^TGCAC
2788 GTGCAC
Asp700I 2635 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5515 GAATACCTTC
AspI 3297 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACACTGTC
BbrPI 820 CACGTG CAC^GTG
1228 CACGTG
BbsI 158 GAAGAC GAAGAC(2/6)
3428 GTCTTC
BcgI 978 GCAGTTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4765 GCACATGTGTCG
BciVI 1677 GGATAC GTATCC(6/5)
852 GTATCC
BglII 378 AGATCT A^GATCT
5310 AGATCT
BpuAI 158 GAAGAC GAAGAC(2/6)
3428 GTCTTC
BspLU11I 1305 ACATGT A^CATGT
4767 ACATGT
BstAPI 2191 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3446 GCAGTCCCTGC
BstEII 638 GGTCACC G^GT[ACGT]ACC
2350 GGTGACC
BstPI 638 GGTCACC G^GT[ACGT]ACC
2350 GGTGACC
DrdI 3522 GACTCGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4323 GACCAGGGGGTC
EcoO65I 638 GGTCACC G^GT[ACGT]ACC
2350 GGTGACC
PflFI 3297 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACACTGTC
PmaCI 820 CACGTG CAC^GTG
1228 CACGTG
PmlI 820 CACGTG CAC^GTG
1228 CACGTG
SbfI 3944 CCTGCAGG CCTGCA^GG
4844 CCTGCAGG
SfiI 187 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3807 GGCCCTGGAGGCC
Sse8387I 3944 CCTGCAGG CCTGCA^GG
4844 CCTGCAGG
Tth111I 3297 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4513 GACACTGTC
XmnI 2635 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5515 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI