##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Aor51HI ApaI AscI Asp718I
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaHI BsiWI
BspDI BspLU11I BssHII Bst1107I BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV FseI
Hin1I KasI KpnI MfeI MluI
MunI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I RsrII SalI
SbfI SfoI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 3667 GTCTAC GT^[AC][GT]AC
AflIII 872 ACACGT A^C[GA][CT]GT
AhdI 3352 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 5152 ATTAAT AT^TAAT
AsnI 5152 ATTAAT AT^TAAT
AspEI 3352 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3382 TGCGCA TGC^GCA
BanI 1987 GGTGCC G^G[CT][GA]CC
BglI 3410 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1371 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3342 GGTCTC GGTCTC(1/5)
BsmBI 4328 CGTCTC CGTCTC(1/5)
BsrDI 14 GCAATG GCAATG(2/0)
BssSI 592 CTCGTG CACGAG(-5/-1)
BstBI 2719 TTCGAA TT^CGAA
DraII 734 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 3352 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 734 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 3382 TGCGCA TGC^GCA
HincII 3577 GTTAAC GT[CT]^[GA]AC
HindII 3577 GTTAAC GT[CT]^[GA]AC
HpaI 3577 GTTAAC GTT^AAC
KspI 3434 CCGCGG CCGC^GG
MamI 1371 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 5405 CATATG CA^TATG
NheI 10 GCTAGC G^CTAGC
NspV 2719 TTCGAA TT^CGAA
PpuMI 734 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2746 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5152 ATTAAT AT^TAAT
PvuI 1014 CGATCG CGAT^CG
SacII 3434 CCGCGG CCGC^GG
ScaI 3392 AGTACT AGT^ACT
SexAI 608 ACCTGGT A^CC[AT]GGT
SfiI 3409 GGCCTGGTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2719 TTCGAA TT^CGAA
SmaI 923 CCCGGG CCC^GGG
SphI 3260 GCATGC GCATG^C
XcmI 497 CCATCCGCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 923 CCCGGG C^CCGGG
XmaI 923 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 3524 TCCGGA T^CCGGA
3899 TCCGGA
Alw44I 784 GTGCAC G^TGCAC
3226 GTGCAC
AlwNI 890 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
5239 CAGATACTG
AocI 4399 CCTCAGG CC^T[ACGT]AGG
4906 CCTGAGG
ApaLI 784 GTGCAC G^TGCAC
3226 GTGCAC
AspI 2269 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3865 GACAGGGTC
AvaI 923 CCCGGG C^[CT]CG[GA]G
3944 CTCGGG
BlpI 139 GCTAAGC GC^T[ACGT]AGC
552 GCTCAGC
Bpu1102I 139 GCTAAGC GC^T[ACGT]AGC
552 GCTCAGC
BsaWI 3524 TCCGGA [AT]^CCGG[AT]
3899 TCCGGA
BseAI 3524 TCCGGA T^CCGGA
3899 TCCGGA
BsiEI 1014 CGATCG CG[GA][CT]^CG
2388 CGACCG
BsmI 1572 GAATGC GAATGC(1/-1)
327 GCATTC
BsoBI 923 CCCGGG C^[CT]CG[GA]G
3944 CTCGGG
BspEI 3524 TCCGGA T^CCGGA
3899 TCCGGA
BsrBI 589 CCGCTC CCGCTC(-3/-3)
4415 GAGCGG
BsrFI 24 GCCGGT [GA]^CCGG[CT]
1984 GCCGGT
BstAPI 557 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
606 GCACCTGGTGC
BstEII 486 GGTGACC G^GT[ACGT]ACC
1308 GGTCACC
BstPI 486 GGTGACC G^GT[ACGT]ACC
1308 GGTCACC
Bsu36I 4399 CCTCAGG CC^T[ACGT]AGG
4906 CCTGAGG
CelII 139 GCTAAGC GC^T[ACGT]AGC
552 GCTCAGC
Cfr10I 24 GCCGGT [GA]^CCGG[CT]
1984 GCCGGT
Eco81I 4399 CCTCAGG CC^T[ACGT]AGG
4906 CCTGAGG
EcoO65I 486 GGTGACC G^GT[ACGT]ACC
1308 GGTCACC
EcoT22I 325 ATGCAT ATGCA^T
5163 ATGCAT
HaeII 531 GGCGCT [GA]GCGC^[CT]
835 GGCGCT
HgaI 4028 GCGTC GACGC(5/10)
4327 GCGTC
HindIII 3691 AAGCTT A^AGCTT
4659 AAGCTT
MroI 3524 TCCGGA T^CCGGA
3899 TCCGGA
NcoI 647 CCATGG C^CATGG
4679 CCATGG
NsiI 325 ATGCAT ATGCA^T
5163 ATGCAT
PflFI 2269 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3865 GACAGGGTC
SacI 2571 GAGCTC GAGCT^C
3439 GAGCTC
SmlI 1623 CTCAAG C^T[CT][GA]AG
2949 CTTGAG
Tth111I 2269 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3865 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BamHI BanII BbsI BbvI
BclI BcnI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraIII DsaI
EaeI EarI EcoNI EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI