##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AatII AflII AgeI Alw44I ApaLI
AscI Asp700I AvrII BfrI BlnI
BsiWI BspDI BspHI Bst1107I BstZ17I
ClaI EcoRV MfeI MluI MunI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmeI PvuI RcaI
SalI SfiI SgrAI SpeI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 4355 GTCTAC GT^[AC][GT]AC
AclI 4914 AACGTT AA^CGTT
AcsI 1541 GAATTC [GA]^AATT[CT]
AhdI 1490 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3357 AGCGCT AGC^GCT
ApoI 1541 GAATTC [GA]^AATT[CT]
AseI 5364 ATTAAT AT^TAAT
AsnI 5364 ATTAAT AT^TAAT
AspEI 1490 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1863 TGCGCA TGC^GCA
BbrPI 2935 CACGTG CAC^GTG
BcgI 3271 GCAGGGCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 339 TGATCA T^GATCA
BsaBI 3832 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1394 GAGACC GGTCTC(1/5)
BsmI 5331 GAATGC GAATGC(1/-1)
BspLU11I 3618 ACATGT A^CATGT
BsrDI 4675 GCAATG GCAATG(2/0)
BstBI 1272 TTCGAA TT^CGAA
CpoI 1377 CGGTCCG CG^G[AT]CCG
Eam1105I 1490 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3357 AGCGCT AGC^GCT
EcoRI 1541 GAATTC G^AATTC
EcoT22I 4140 ATGCAT ATGCA^T
FbaI 339 TGATCA T^GATCA
FseI 4813 GGCCGGCC GGCCGG^CC
FspI 1863 TGCGCA TGC^GCA
HindIII 551 AAGCTT A^AGCTT
HpaI 5273 GTTAAC GTT^AAC
MamI 3832 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 4140 ATGCAT ATGCA^T
NspV 1272 TTCGAA TT^CGAA
PmaCI 2935 CACGTG CAC^GTG
PmlI 2935 CACGTG CAC^GTG
PshAI 10 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 5364 ATTAAT AT^TAAT
Psp1406I 4914 AACGTT AA^CGTT
RsrII 1377 CGGTCCG CG^G[AT]CCG
SapI 4906 GAAGAGC GCTCTTC(1/4)
ScaI 1476 AGTACT AGT^ACT
SfuI 1272 TTCGAA TT^CGAA
SnaBI 5254 TACGTA TAC^GTA
SphI 2444 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 255 GGTACC G^GTACC
2064 GGTACC
AccIII 2035 TCCGGA T^CCGGA
4460 TCCGGA
Asp718I 255 GGTACC G^GTACC
2064 GGTACC
AspI 4021 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5230 GACGGCGTC
BalI 162 TGGCCA TGG^CCA
2085 TGGCCA
BamHI 1798 GGATCC G^GATCC
3476 GGATCC
BbeI 2126 GGCGCC GGCGC^C
4094 GGCGCC
BseAI 2035 TCCGGA T^CCGGA
4460 TCCGGA
BsiEI 2261 CGGCCG CG[GA][CT]^CG
4812 CGGCCG
Bsp1407I 3307 TGTACA T^GTACA
5380 TGTACA
BspEI 2035 TCCGGA T^CCGGA
4460 TCCGGA
BsrBI 4388 CCGCTC CCGCTC(-3/-3)
2258 GAGCGG
BsrGI 3307 TGTACA T^GTACA
5380 TGTACA
DraIII 135 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1817 CACATCGTG
DrdI 175 GACTAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1645 GACGCGGGAGTC
EagI 2261 CGGCCG C^GGCCG
4812 CGGCCG
EclXI 2261 CGGCCG C^GGCCG
4812 CGGCCG
Eco52I 2261 CGGCCG C^GGCCG
4812 CGGCCG
KasI 2126 GGCGCC G^GCGCC
4094 GGCGCC
KpnI 255 GGTACC GGTAC^C
2064 GGTACC
KspI 3638 CCGCGG CCGC^GG
5209 CCGCGG
MluNI 162 TGGCCA TGG^CCA
2085 TGGCCA
MroI 2035 TCCGGA T^CCGGA
4460 TCCGGA
MscI 162 TGGCCA TGG^CCA
2085 TGGCCA
NarI 2126 GGCGCC GG^CGCC
4094 GGCGCC
NspI 2444 GCATGC [GA]CATG^[CT]
3618 ACATGT
PflFI 4021 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5230 GACGGCGTC
SacII 3638 CCGCGG CCGC^GG
5209 CCGCGG
SbfI 1339 CCTGCAGG CCTGCA^GG
3268 CCTGCAGG
SfoI 2126 GGCGCC GGC^GCC
4094 GGCGCC
Sse8387I 1339 CCTGCAGG CCTGCA^GG
3268 CCTGCAGG
SspBI 3307 TGTACA T^GTACA
5380 TGTACA
TfiI 1430 GATTC G^A[AT]TC
4148 GAATC
Tth111I 4021 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5230 GACGGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI