##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AvrII
BciVI BfrI BglII BlnI BseAI
BsiWI BsmBI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoRV FseI
HgaI HincII HindII HpaI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SmaI SnaBI SpeI SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1225 GGTACC G^GTACC
AcyI 67 GGCGCC G[GA]^CG[CT]C
ApaI 1014 GGGCCC GGGCC^C
Asp700I 4460 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1225 GGTACC G^GTACC
AviII 5173 TGCGCA TGC^GCA
BamHI 2165 GGATCC G^GATCC
BbeI 67 GGCGCC GGCGC^C
BbrPI 3841 CACGTG CAC^GTG
BglI 2173 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3641 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BsaWI 664 TCCGGT [AT]^CCGG[AT]
BsiEI 2826 CGGCCG CG[GA][CT]^CG
BspMI 1035 GCAGGT ACCTGC(4/8)
BsrFI 2828 GCCGGC [GA]^CCGG[CT]
BssSI 1164 CACGAG CACGAG(-5/-1)
BstAPI 2251 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1140 GGTGACC G^GT[ACGT]ACC
BstPI 1140 GGTGACC G^GT[ACGT]ACC
Cfr10I 2828 GCCGGC [GA]^CCGG[CT]
DraI 4585 TTTAAA TTT^AAA
EagI 2826 CGGCCG C^GGCCG
EclXI 2826 CGGCCG C^GGCCG
Eco52I 2826 CGGCCG C^GGCCG
EcoO65I 1140 GGTGACC G^GT[ACGT]ACC
FspI 5173 TGCGCA TGC^GCA
Hin1I 67 GGCGCC G[GA]^CG[CT]C
KasI 67 GGCGCC G^GCGCC
KpnI 1225 GGTACC GGTAC^C
KspI 2823 CCGCGG CCGC^GG
MamI 3641 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2828 GCCGGC GCC^GGC
NarI 67 GGCGCC GG^CGCC
NdeI 1949 CATATG CA^TATG
NgoMIV 2828 GCCGGC G^CCGGC
PmaCI 3841 CACGTG CAC^GTG
PmlI 3841 CACGTG CAC^GTG
SacII 2823 CCGCGG CCGC^GG
SapI 2790 GCTCTTC GCTCTTC(1/4)
SbfI 1032 CCTGCAGG CCTGCA^GG
ScaI 4901 AGTACT AGT^ACT
SexAI 631 ACCAGGT A^CC[AT]GGT
SfiI 2172 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 67 GGCGCC GGC^GCC
SphI 2655 GCATGC GCATG^C
Sse8387I 1032 CCTGCAGG CCTGCA^GG
XbaI 230 TCTAGA T^CTAGA
XmnI 4460 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 80 CGCG CG^CG
2824 CGCG
BclI 4052 TGATCA T^GATCA
4104 TGATCA
BlpI 917 GCTCAGC GC^T[ACGT]AGC
2832 GCTGAGC
Bpu1102I 917 GCTCAGC GC^T[ACGT]AGC
2832 GCTGAGC
BsaAI 3841 CACGTG [CT]AC^GT[GA]
4833 CACGTA
BsaI 1684 GAGACC GGTCTC(1/5)
3779 GAGACC
BsmI 2244 GAATGC GAATGC(1/-1)
3194 GAATGC
BspHI 1670 TCATGA T^CATGA
4325 TCATGA
BspLU11I 4057 ACATGT A^CATGT
4967 ACATGT
BsrDI 5157 GCAATG GCAATG(2/0)
5227 GCAATG
BstUI 80 CGCG CG^CG
2824 CGCG
CelII 917 GCTCAGC GC^T[ACGT]AGC
2832 GCTGAGC
DraIII 630 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3214 CACATGGTG
EcoNI 1877 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4450 CCTAGCATAGG
EcoT22I 4500 ATGCAT ATGCA^T
4540 ATGCAT
FbaI 4052 TGATCA T^GATCA
4104 TGATCA
MvnI 80 CGCG CG^CG
2824 CGCG
NsiI 4500 ATGCAT ATGCA^T
4540 ATGCAT
PflMI 1163 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1460 CCAGCAAATGG
RcaI 1670 TCATGA T^CATGA
4325 TCATGA
SmlI 571 CTCAAG C^T[CT][GA]AG
3433 CTTGAG
Van91I 1163 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1460 CCAGCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrBI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII