##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BlnI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I Eco47III EcoRV
FseI HindIII HpaI KpnI KspI
MfeI MunI NdeI NheI NotI
NspV PacI PflFI PinAI PmeI
PshBI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1701 GTAGAC GT^[AC][GT]AC
AclI 1870 AACGTT AA^CGTT
AflII 3269 CTTAAG C^TTAAG
AviII 292 TGCGCA TGC^GCA
BclI 2688 TGATCA T^GATCA
BfrI 3269 CTTAAG C^TTAAG
BglII 1036 AGATCT A^GATCT
BsaBI 1244 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1057 ACATGT A^CATGT
EagI 2478 CGGCCG C^GGCCG
EclXI 2478 CGGCCG C^GGCCG
Eco52I 2478 CGGCCG C^GGCCG
EcoRI 1837 GAATTC G^AATTC
EcoT22I 3335 ATGCAT ATGCA^T
FbaI 2688 TGATCA T^GATCA
FspI 292 TGCGCA TGC^GCA
MamI 1244 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 618 ACGCGT A^CGCGT
NaeI 2938 GCCGGC GCC^GGC
NgoMIV 2938 GCCGGC G^CCGGC
NruI 1375 TCGCGA TCG^CGA
NsiI 3335 ATGCAT ATGCA^T
NspI 1057 ACATGT [GA]CATG^[CT]
PaeR7I 1978 CTCGAG C^TCGAG
PflMI 841 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1139 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1870 AACGTT AA^CGTT
PvuI 4392 CGATCG CGAT^CG
SbfI 2093 CCTGCAGG CCTGCA^GG
SgrAI 2489 CACCGGCG C[GA]^CCGG[CT]G
SmaI 571 CCCGGG CCC^GGG
Sse8387I 2093 CCTGCAGG CCTGCA^GG
SspI 5029 AATATT AAT^ATT
Van91I 841 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1978 CTCGAG C^TCGAG
XmaCI 571 CCCGGG C^CCGGG
XmaI 571 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 2544 TCCGGA T^CCGGA
2616 TCCGGA
AcsI 1837 GAATTC [GA]^AATT[CT]
4034 AAATTC
AocI 1202 CCTGAGG CC^T[ACGT]AGG
2052 CCTGAGG
ApoI 1837 GAATTC [GA]^AATT[CT]
4034 AAATTC
Asp700I 470 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
857 GAAAATGTTC
BbrPI 3019 CACGTG CAC^GTG
3101 CACGTG
BciVI 2654 GGATAC GTATCC(6/5)
2746 GTATCC
BlpI 681 GCTAAGC GC^T[ACGT]AGC
3421 GCTCAGC
Bpu1102I 681 GCTAAGC GC^T[ACGT]AGC
3421 GCTCAGC
BseAI 2544 TCCGGA T^CCGGA
2616 TCCGGA
BspEI 2544 TCCGGA T^CCGGA
2616 TCCGGA
BsrBI 2309 CCGCTC CCGCTC(-3/-3)
3419 CCGCTC
BsrFI 2490 ACCGGC [GA]^CCGG[CT]
2938 GCCGGC
BstAPI 4755 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4782 GCATTTTGTGC
BstEII 2279 GGTCACC G^GT[ACGT]ACC
4368 GGTAACC
BstPI 2279 GGTCACC G^GT[ACGT]ACC
4368 GGTAACC
BstYI 1036 AGATCT [GA]^GATC[CT]
4727 GGATCT
Bsu36I 1202 CCTGAGG CC^T[ACGT]AGG
2052 CCTGAGG
CelII 681 GCTAAGC GC^T[ACGT]AGC
3421 GCTCAGC
Cfr10I 2490 ACCGGC [GA]^CCGG[CT]
2938 GCCGGC
DraI 1292 TTTAAA TTT^AAA
1307 TTTAAA
DraIII 3068 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4319 CACCATGTG
DrdI 984 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4279 GACCGACCAGTC
Eco81I 1202 CCTGAGG CC^T[ACGT]AGG
2052 CCTGAGG
EcoNI 2836 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2878 CCTCTTCAAGG
EcoO65I 2279 GGTCACC G^GT[ACGT]ACC
4368 GGTAACC
HincII 4239 GTCAAC GT[CT]^[GA]AC
5037 GTCAAC
HindII 4239 GTCAAC GT[CT]^[GA]AC
5037 GTCAAC
MflI 1036 AGATCT [GA]^GATC[CT]
4727 GGATCT
MroI 2544 TCCGGA T^CCGGA
2616 TCCGGA
NcoI 638 CCATGG C^CATGG
1994 CCATGG
PmaCI 3019 CACGTG CAC^GTG
3101 CACGTG
PmlI 3019 CACGTG CAC^GTG
3101 CACGTG
SapI 1194 GAAGAGC GCTCTTC(1/4)
4131 GCTCTTC
XhoII 1036 AGATCT [GA]^GATC[CT]
4727 GGATCT
XmnI 470 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
857 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI