##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I BbeI
BbrPI BciVI BclI BsaAI BsiEI
BsiWI Bsp1407I BspDI BspHI BsrGI
BssHII BstBI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HpaI KasI KpnI
KspI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1061 GACGTC GACGT^C
AccIII 501 TCCGGA T^CCGGA
AcyI 1061 GACGTC G[GA]^CG[CT]C
AflII 1587 CTTAAG C^TTAAG
AflIII 3310 ACATGT A^C[GA][CT]GT
Alw44I 1674 GTGCAC G^TGCAC
ApaLI 1674 GTGCAC G^TGCAC
AspI 3842 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4310 TGCGCA TGC^GCA
BamHI 1188 GGATCC G^GATCC
BbvCI 2459 CCTCAGC CCTCAGC(-5/-2)
BfrI 1587 CTTAAG C^TTAAG
BglI 566 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1061 GACGTC G[GA]^CG[CT]C
BseAI 501 TCCGGA T^CCGGA
BsmBI 3374 CGTCTC CGTCTC(1/5)
BspEI 501 TCCGGA T^CCGGA
BspLU11I 3310 ACATGT A^CATGT
BsrFI 4231 GCCGGC [GA]^CCGG[CT]
Bst1107I 1763 GTATAC GTA^TAC
BstEII 2212 GGTGACC G^GT[ACGT]ACC
BstPI 2212 GGTGACC G^GT[ACGT]ACC
BstXI 3144 CCAAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1763 GTATAC GTA^TAC
Cfr10I 4231 GCCGGC [GA]^CCGG[CT]
DraI 3824 TTTAAA TTT^AAA
DrdI 1532 GACAACTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4350 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2212 GGTGACC G^GT[ACGT]ACC
EcoRI 1410 GAATTC G^AATTC
FauI 1185 GCGGG CCCGC(4/6)
FspI 4310 TGCGCA TGC^GCA
HaeII 2037 AGCGCC [GA]GCGC^[CT]
HgaI 4421 GCGTC GACGC(5/10)
Hin1I 1061 GACGTC G[GA]^CG[CT]C
HindIII 3542 AAGCTT A^AGCTT
MfeI 3938 CAATTG C^AATTG
MroI 501 TCCGGA T^CCGGA
MunI 3938 CAATTG C^AATTG
NaeI 4231 GCCGGC GCC^GGC
NgoMIV 4231 GCCGGC G^CCGGC
PflFI 3842 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1000 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 2465 CCCGGG CCC^GGG
SpeI 3230 ACTAGT A^CTAGT
Tth111I 3842 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2465 CCCGGG C^CCGGG
XmaI 2465 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 31 CGCG CG^CG
264 CGCG
AcsI 1410 GAATTC [GA]^AATT[CT]
5146 GAATTT
ApoI 1410 GAATTC [GA]^AATT[CT]
5146 GAATTT
AvrII 2898 CCTAGG C^CTAGG
4761 CCTAGG
BlnI 2898 CCTAGG C^CTAGG
4761 CCTAGG
BsaBI 2284 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5010 GATGGCCATC
BsaI 643 GAGACC GGTCTC(1/5)
4163 GGTCTC
BsaWI 501 TCCGGA [AT]^CCGG[AT]
1418 TCCGGT
BsrBI 2631 CCGCTC CCGCTC(-3/-3)
25 GAGCGG
BsrDI 823 CATTGC GCAATG(2/0)
3891 CATTGC
BssSI 112 CTCGTG CACGAG(-5/-1)
2728 CTCGTG
BstUI 31 CGCG CG^CG
264 CGCG
MamI 2284 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5010 GATGGCCATC
MvnI 31 CGCG CG^CG
264 CGCG
NcoI 1875 CCATGG C^CATGG
4268 CCATGG
PpuMI 464 AGGTCCC [GA]G^G[AT]CC[CT]
4787 AGGACCC
PvuII 3570 CAGCTG CAG^CTG
4973 CAGCTG
SacI 52 GAGCTC GAGCT^C
2476 GAGCTC
SapI 2889 GCTCTTC GCTCTTC(1/4)
5002 GCTCTTC
SexAI 215 ACCAGGT A^CC[AT]GGT
4538 ACCAGGT
SspI 496 AATATT AAT^ATT
1941 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII