##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI Asp700I AviII BciVI
BclI BfrI BsiWI Bsp1407I BspDI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FbaI FspI HpaI MfeI MluI
MunI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 87 TCCGGA T^CCGGA
AcsI 5346 AAATTT [GA]^AATT[CT]
AhdI 4450 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3020 GTGCAC G^TGCAC
Aor51HI 3949 AGCGCT AGC^GCT
ApaLI 3020 GTGCAC G^TGCAC
ApoI 5346 AAATTT [GA]^AATT[CT]
AspEI 4450 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4363 CCTAGG C^CTAGG
BbrPI 4833 CACGTG CAC^GTG
BcgI 3333 GCAACTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 4364 CTAG C^TAG
BlnI 4363 CCTAGG C^CTAGG
BsaWI 87 TCCGGA [AT]^CCGG[AT]
BseAI 87 TCCGGA T^CCGGA
BspEI 87 TCCGGA T^CCGGA
BspLU11I 2004 ACATGT A^CATGT
BssHII 2731 GCGCGC G^CGCGC
BstAPI 703 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 314 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4450 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3949 AGCGCT AGC^GCT
EcoNI 1874 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 1894 GGCCGGCC GGCCGG^CC
HindIII 3074 AAGCTT A^AGCTT
KspI 3554 CCGCGG CCGC^GG
MaeI 4364 CTAG C^TAG
MroI 87 TCCGGA T^CCGGA
NcoI 4329 CCATGG C^CATGG
NruI 1059 TCGCGA TCG^CGA
PmaCI 4833 CACGTG CAC^GTG
PmlI 4833 CACGTG CAC^GTG
SacII 3554 CCGCGG CCGC^GG
SalI 4632 GTCGAC G^TCGAC
SexAI 3348 ACCTGGT A^CC[AT]GGT
SgrAI 3574 CGCCGGTG C[GA]^CCGG[CT]G
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2232 GGTACC G^GTACC
3926 GGTACC
Asp718I 2232 GGTACC G^GTACC
3926 GGTACC
BamHI 90 GGATCC G^GATCC
303 GGATCC
BglII 2178 AGATCT A^GATCT
2715 AGATCT
BsaAI 2762 TACGTG [CT]AC^GT[GA]
4833 CACGTG
BsaBI 127 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3298 GATGGCCATC
BsmI 3537 GAATGC GAATGC(1/-1)
634 GCATTC
BsrBI 3376 CCGCTC CCGCTC(-3/-3)
2137 GAGCGG
BsrDI 4532 CATTGC GCAATG(2/0)
4550 CATTGC
KpnI 2232 GGTACC GGTAC^C
3926 GGTACC
MamI 127 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3298 GATGGCCATC
NspI 2004 ACATGT [GA]CATG^[CT]
2094 ACATGC
PshAI 3031 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3749 GACATCGGTC
PvuII 45 CAGCTG CAG^CTG
4232 CAGCTG
SacI 242 GAGCTC GAGCT^C
3422 GAGCTC
SapI 2947 GCTCTTC GCTCTTC(1/4)
3664 GCTCTTC
SmaI 3215 CCCGGG CCC^GGG
3411 CCCGGG
Tsp509I 5347 AATT ^AATT
5472 AATT
XmaCI 3215 CCCGGG C^CCGGG
3411 CCCGGG
XmaI 3215 CCCGGG C^CCGGG
3411 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoII