##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII Acc65I AccI AclI AscI
AseI AsnI Asp718I AspI BamHI
BcgI BsiWI Bsp1407I BspDI BsrBI
BsrGI BssHII Bst1107I BstAPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FseI HpaI KpnI
MfeI MluI MunI NcoI NdeI
NotI NruI PacI PflFI PmeI
PshBI Psp1406I PvuI RsrII SalI
ScaI SexAI SfiI SnaBI SpeI
SphI SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 3376 TCCGGA T^CCGGA
AflII 1758 CTTAAG C^TTAAG
AgeI 1094 ACCGGT A^CCGGT
Aor51HI 4561 AGCGCT AGC^GCT
Asp700I 470 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4412 TGCGCA TGC^GCA
AvrII 5054 CCTAGG C^CTAGG
BbrPI 2249 CACGTG CAC^GTG
BciVI 4620 GTATCC GTATCC(6/5)
BfrI 1758 CTTAAG C^TTAAG
BglII 5476 AGATCT A^GATCT
BlnI 5054 CCTAGG C^CTAGG
BsaBI 4299 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3376 TCCGGA T^CCGGA
BsiEI 5163 CGACCG CG[GA][CT]^CG
BsmI 615 GCATTC GAATGC(1/-1)
BspEI 3376 TCCGGA T^CCGGA
BspLU11I 2926 ACATGT A^CATGT
BstBI 2788 TTCGAA TT^CGAA
DraI 4037 TTTAAA TTT^AAA
DraIII 1336 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4561 AGCGCT AGC^GCT
EcoNI 4334 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5070 GAATTC G^AATTC
FspI 4412 TGCGCA TGC^GCA
KspI 4805 CCGCGG CCGC^GG
MamI 4299 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3376 TCCGGA T^CCGGA
NaeI 2384 GCCGGC GCC^GGC
NgoMIV 2384 GCCGGC G^CCGGC
NheI 1436 GCTAGC G^CTAGC
NspV 2788 TTCGAA TT^CGAA
PinAI 1094 ACCGGT A^CCGGT
PmaCI 2249 CACGTG CAC^GTG
PmlI 2249 CACGTG CAC^GTG
PshAI 1416 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4805 CCGCGG CCGC^GG
SbfI 1409 CCTGCAGG CCTGCA^GG
SfuI 2788 TTCGAA TT^CGAA
SgrAI 1093 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 1409 CCTGCAGG CCTGCA^GG
SspI 2391 AATATT AAT^ATT
StuI 4968 AGGCCT AGG^CCT
XmnI 470 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 3631 CGCG CG^CG
4806 CGCG
AcsI 4190 AAATTC [GA]^AATT[CT]
5070 GAATTC
AflIII 2250 ACGTGT A^C[GA][CT]GT
2926 ACATGT
AhdI 2513 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACAAGAGGTC
Alw44I 1905 GTGCAC G^TGCAC
2598 GTGCAC
ApaI 4809 GGGCCC GGGCC^C
5428 GGGCCC
ApaLI 1905 GTGCAC G^TGCAC
2598 GTGCAC
ApoI 4190 AAATTC [GA]^AATT[CT]
5070 GAATTC
AspEI 2513 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACAAGAGGTC
BbeI 2528 GGCGCC GGCGC^C
5376 GGCGCC
BsaAI 2106 TACGTG [CT]AC^GT[GA]
2249 CACGTG
BsaI 4464 GAGACC GGTCTC(1/5)
1948 GGTCTC
BspHI 4436 TCATGA T^CATGA
5408 TCATGA
BssSI 3555 CACGAG CACGAG(-5/-1)
4424 CACGAG
BstEII 530 GGTTACC G^GT[ACGT]ACC
3728 GGTAACC
BstPI 530 GGTTACC G^GT[ACGT]ACC
3728 GGTAACC
BstUI 3631 CGCG CG^CG
4806 CGCG
Eam1105I 2513 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5347 GACAAGAGGTC
EcoO65I 530 GGTTACC G^GT[ACGT]ACC
3728 GGTAACC
EcoT22I 613 ATGCAT ATGCA^T
1468 ATGCAT
HgaI 2472 GCGTC GACGC(5/10)
3749 GCGTC
KasI 2528 GGCGCC G^GCGCC
5376 GGCGCC
MvnI 3631 CGCG CG^CG
4806 CGCG
NarI 2528 GGCGCC GG^CGCC
5376 GGCGCC
NsiI 613 ATGCAT ATGCA^T
1468 ATGCAT
NspI 19 GCATGT [GA]CATG^[CT]
2926 ACATGT
PaeR7I 3223 CTCGAG C^TCGAG
4862 CTCGAG
RcaI 4436 TCATGA T^CATGA
5408 TCATGA
SfoI 2528 GGCGCC GGC^GCC
5376 GGCGCC
XhoI 3223 CTCGAG C^TCGAG
4862 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI