##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I ApaLI
ApoI AseI AsnI Asp700I Asp718I
BamHI BbrPI BbsI BcgI BfrI
BglII BpuAI BsaBI BseAI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstZ17I CpoI DraI
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI MamI MluI MroI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SfuI SnaBI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 3484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 1832 GGCGCGCC GG^CGCGCC
AspEI 3484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1587 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1079 GGCGCC GGCGC^C
BciVI 74 GGATAC GTATCC(6/5)
BclI 2778 TGATCA T^GATCA
BsiWI 1898 CGTACG C^GTACG
BspDI 3969 ATCGAT AT^CGAT
BsrDI 3111 CATTGC GCAATG(2/0)
BstEII 337 GGTCACC G^GT[ACGT]ACC
BstPI 337 GGTCACC G^GT[ACGT]ACC
BstXI 4120 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3969 ATCGAT AT^CGAT
DrdI 1807 GACGCCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3484 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 337 GGTCACC G^GT[ACGT]ACC
FbaI 2778 TGATCA T^GATCA
HincII 2695 GTCGAC GT[CT]^[GA]AC
HindII 2695 GTCGAC GT[CT]^[GA]AC
KasI 1079 GGCGCC G^GCGCC
MfeI 5054 CAATTG C^AATTG
MunI 5054 CAATTG C^AATTG
NarI 1079 GGCGCC GG^CGCC
NcoI 4034 CCATGG C^CATGG
NdeI 37 CATATG CA^TATG
PflFI 1587 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 2695 GTCGAC G^TCGAC
SbfI 1658 CCTGCAGG CCTGCA^GG
SfiI 2962 GGCCACCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1079 GGCGCC GGC^GCC
SgrAI 1483 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 428 ACTAGT A^CTAGT
SphI 3575 GCATGC GCATG^C
Sse8387I 1658 CCTGCAGG CCTGCA^GG
Tth111I 1587 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AviII 3425 TGCGCA TGC^GCA
3943 TGCGCA
AvrII 4256 CCTAGG C^CTAGG
5040 CCTAGG
BbvCI 420 GCTGAGG CCTCAGC(-5/-2)
1311 GCTGAGG
BlnI 4256 CCTAGG C^CTAGG
5040 CCTAGG
BlpI 1649 GCTCAGC GC^T[ACGT]AGC
4448 GCTGAGC
Bpu1102I 1649 GCTCAGC GC^T[ACGT]AGC
4448 GCTGAGC
BsaWI 1934 TCCGGT [AT]^CCGG[AT]
3547 ACCGGA
BsiEI 1402 CGGCCG CG[GA][CT]^CG
3675 CGGCCG
BsmBI 1592 CGTCTC CGTCTC(1/5)
2559 GAGACG
BsrBI 1953 GAGCGG CCGCTC(-3/-3)
1959 GAGCGG
BstAPI 2303 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3713 GCACAGCGTGC
CelII 1649 GCTCAGC GC^T[ACGT]AGC
4448 GCTGAGC
EagI 1402 CGGCCG C^GGCCG
3675 CGGCCG
EclXI 1402 CGGCCG C^GGCCG
3675 CGGCCG
Eco52I 1402 CGGCCG C^GGCCG
3675 CGGCCG
FspI 3425 TGCGCA TGC^GCA
3943 TGCGCA
KspI 1376 CCGCGG CCGC^GG
1841 CCGCGG
SacII 1376 CCGCGG CCGC^GG
1841 CCGCGG
SapI 2186 GCTCTTC GCTCTTC(1/4)
3806 GCTCTTC
SexAI 3919 ACCAGGT A^CC[AT]GGT
5114 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI