##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspHI AviII AvrII
BbeI BbrPI BcgI BlnI BsaWI
BseAI BsiHKAI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 1504 CGCG CG^CG
AcyI 1033 GACGCC G[GA]^CG[CT]C
AflII 1723 CTTAAG C^TTAAG
Aor51HI 1751 AGCGCT AGC^GCT
AseI 897 ATTAAT AT^TAAT
AsnI 897 ATTAAT AT^TAAT
Asp700I 2791 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1426 GGATCC G^GATCC
BciVI 3045 GGATAC GTATCC(6/5)
BclI 3276 TGATCA T^GATCA
BfrI 1723 CTTAAG C^TTAAG
BlpI 4316 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4316 GCTTAGC GC^T[ACGT]AGC
BsaAI 2268 CACGTA [CT]AC^GT[GA]
BsaHI 1033 GACGCC G[GA]^CG[CT]C
BsiEI 3744 CGACCG CG[GA][CT]^CG
BspHI 2487 TCATGA T^CATGA
BsrFI 4908 GCCGGT [GA]^CCGG[CT]
BssSI 4633 CTCGTG CACGAG(-5/-1)
BstUI 1504 CGCG CG^CG
CelII 4316 GCTTAGC GC^T[ACGT]AGC
Cfr10I 4908 GCCGGT [GA]^CCGG[CT]
DraI 375 TTTAAA TTT^AAA
DrdI 962 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 915 GAAGAG CTCTTC(1/4)
Eco47III 1751 AGCGCT AGC^GCT
EcoRV 2046 GATATC GAT^ATC
EcoT22I 1762 ATGCAT ATGCA^T
FbaI 3276 TGATCA T^GATCA
Hin1I 1033 GACGCC G[GA]^CG[CT]C
HincII 5300 GTTGAC GT[CT]^[GA]AC
HindII 5300 GTTGAC GT[CT]^[GA]AC
Ksp632I 915 GAAGAG CTCTTC(1/4)
MvnI 1504 CGCG CG^CG
NdeI 3379 CATATG CA^TATG
NsiI 1762 ATGCAT ATGCA^T
PshBI 897 ATTAAT AT^TAAT
RcaI 2487 TCATGA T^CATGA
XbaI 5282 TCTAGA T^CTAGA
XmnI 2791 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 1740 GGTGCC G^G[CT][GA]CC
4965 GGTGCC
BbsI 204 GAAGAC GAAGAC(2/6)
3635 GTCTTC
BglII 247 AGATCT A^GATCT
2922 AGATCT
BpuAI 204 GAAGAC GAAGAC(2/6)
3635 GTCTTC
BsaBI 2067 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5164 GATATATATC
BsrDI 2597 CATTGC GCAATG(2/0)
4607 CATTGC
BstAPI 1460 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3541 GCAACCTCTGC
EcoRI 3758 GAATTC G^AATTC
4843 GAATTC
HaeII 210 AGCGCC [GA]GCGC^[CT]
1751 AGCGCT
HgaI 1033 GACGC GACGC(5/10)
2639 GCGTC
MamI 2067 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5164 GATATATATC
ScaI 1712 AGTACT AGT^ACT
3073 AGTACT
SfiI 575 GGCCTTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
749 GGCCAAAATGGCC
SmaI 3110 CCCGGG CCC^GGG
4714 CCCGGG
SphI 2094 GCATGC GCATG^C
5314 GCATGC
SspI 1656 AATATT AAT^ATT
2348 AATATT
XcmI 595 CCACTACTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4848 CCATTTACCTTCTGG
XmaCI 3110 CCCGGG C^CCGGG
4714 CCCGGG
XmaI 3110 CCCGGG C^CCGGG
4714 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspI AvaI AvaII BalI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII