##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BciVI
BclI BsaBI BsiWI BspDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 226 TCCGGA T^CCGGA
AflII 805 CTTAAG C^TTAAG
AflIII 3384 ACATGT A^C[GA][CT]GT
AspI 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3765 TGCGCA TGC^GCA
AvrII 3650 CCTAGG C^CTAGG
BamHI 880 GGATCC G^GATCC
BfrI 805 CTTAAG C^TTAAG
BglII 4217 AGATCT A^GATCT
BlnI 3650 CCTAGG C^CTAGG
BseAI 226 TCCGGA T^CCGGA
BsmBI 770 GAGACG CGTCTC(1/5)
BspEI 226 TCCGGA T^CCGGA
BspLU11I 3384 ACATGT A^CATGT
DrdI 4095 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3765 TGCGCA TGC^GCA
MroI 226 TCCGGA T^CCGGA
NaeI 2253 GCCGGC GCC^GGC
NgoMIV 2253 GCCGGC G^CCGGC
NotI 5822 GCGGCCGC GC^GGCCGC
PaeR7I 2828 CTCGAG C^TCGAG
PflFI 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 230 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 493 GAAGAGC GCTCTTC(1/4)
SbfI 2009 CCTGCAGG CCTGCA^GG
SfiI 61 GGCCCTGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2009 CCTGCAGG CCTGCA^GG
Tsp509I 591 AATT ^AATT
Tth111I 219 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2828 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1888 GTGCAC G^TGCAC
2055 GTGCAC
ApaLI 1888 GTGCAC G^TGCAC
2055 GTGCAC
BbvCI 1226 GCTGAGG CCTCAGC(-5/-2)
2261 GCTGAGG
BcgI 4618 CGAAACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5506 CGAGCCAGGTGC
BsiEI 4687 CGGCCG CG[GA][CT]^CG
5823 CGGCCG
BspHI 238 TCATGA T^CATGA
2452 TCATGA
BsrDI 2230 CATTGC GCAATG(2/0)
3470 CATTGC
BssSI 818 CACGAG CACGAG(-5/-1)
4778 CACGAG
BstEII 3428 GGTAACC G^GT[ACGT]ACC
5485 GGTGACC
BstPI 3428 GGTAACC G^GT[ACGT]ACC
5485 GGTGACC
EagI 4687 CGGCCG C^GGCCG
5823 CGGCCG
EclXI 4687 CGGCCG C^GGCCG
5823 CGGCCG
Eco52I 4687 CGGCCG C^GGCCG
5823 CGGCCG
EcoO65I 3428 GGTAACC G^GT[ACGT]ACC
5485 GGTGACC
EcoT22I 3190 ATGCAT ATGCA^T
5082 ATGCAT
NsiI 3190 ATGCAT ATGCA^T
5082 ATGCAT
RcaI 238 TCATGA T^CATGA
2452 TCATGA
TfiI 3522 GATTC G^A[AT]TC
4028 GATTC
XbaI 3824 TCTAGA T^CTAGA
4996 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmaI SmlI SphI SspBI StuI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI