##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AspI AviII AvrII BcgI BclI
BfrI BlnI BsaBI BseAI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI MamI MfeI
MluI MroI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 458 GACGTC GACGT^C
AcsI 1872 GAATTT [GA]^AATT[CT]
AflIII 5288 ACATGT A^C[GA][CT]GT
AhdI 1453 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2883 CCTCAGG CC^T[ACGT]AGG
Aor51HI 749 AGCGCT AGC^GCT
ApoI 1872 GAATTT [GA]^AATT[CT]
Asp700I 4491 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1453 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 47 GGATCC G^GATCC
BbeI 3411 GGCGCC GGCGC^C
BglII 1745 AGATCT A^GATCT
BsaWI 2626 ACCGGA [AT]^CCGG[AT]
BsiEI 3425 CGGCCG CG[GA][CT]^CG
BsmI 5176 GCATTC GAATGC(1/-1)
Bsp1407I 933 TGTACA T^GTACA
BspLU11I 5288 ACATGT A^CATGT
BsrDI 1844 GCAATG GCAATG(2/0)
BsrGI 933 TGTACA T^GTACA
Bsu36I 2883 CCTCAGG CC^T[ACGT]AGG
DraI 1940 TTTAAA TTT^AAA
EagI 3425 CGGCCG C^GGCCG
Eam1105I 1453 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3425 CGGCCG C^GGCCG
Eco47III 749 AGCGCT AGC^GCT
Eco52I 3425 CGGCCG C^GGCCG
Eco81I 2883 CCTCAGG CC^T[ACGT]AGG
HincII 3970 GTCAAC GT[CT]^[GA]AC
HindII 3970 GTCAAC GT[CT]^[GA]AC
HindIII 1936 AAGCTT A^AGCTT
KasI 3411 GGCGCC G^GCGCC
KspI 3336 CCGCGG CCGC^GG
NaeI 3427 GCCGGC GCC^GGC
NarI 3411 GGCGCC GG^CGCC
NgoMIV 3427 GCCGGC G^CCGGC
SacII 3336 CCGCGG CCGC^GG
SfoI 3411 GGCGCC GGC^GCC
SpeI 1770 ACTAGT A^CTAGT
SspBI 933 TGTACA T^GTACA
SspI 1669 AATATT AAT^ATT
XmnI 4491 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 1203 GTGCAC G^TGCAC
1805 GTGCAC
ApaLI 1203 GTGCAC G^TGCAC
1805 GTGCAC
BbrPI 3184 CACGTG CAC^GTG
3214 CACGTG
BciVI 2629 GGATAC GTATCC(6/5)
2406 GTATCC
BsrFI 3427 GCCGGC [GA]^CCGG[CT]
3678 ACCGGC
BstEII 3929 GGTTACC G^GT[ACGT]ACC
5301 GGTGACC
BstPI 3929 GGTTACC G^GT[ACGT]ACC
5301 GGTGACC
Cfr10I 3427 GCCGGC [GA]^CCGG[CT]
3678 ACCGGC
DraIII 1220 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
5188 CACACTGTG
EcoO65I 3929 GGTTACC G^GT[ACGT]ACC
5301 GGTGACC
HaeII 749 AGCGCT [GA]GCGC^[CT]
3411 GGCGCC
NdeI 2059 CATATG CA^TATG
5318 CATATG
PmaCI 3184 CACGTG CAC^GTG
3214 CACGTG
PmlI 3184 CACGTG CAC^GTG
3214 CACGTG
SbfI 51 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
Sse8387I 51 CCTGCAGG CCTGCA^GG
390 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI