##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI AspI BciVI BfrI
BsaBI BsiWI BspDI BspHI BspLU11I
BstBI ClaI DraI EcoRV KspI
MamI MfeI MluI MunI NdeI
NheI NotI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I RcaI
SacII SalI SbfI ScaI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 5216 GTATAC GT^[AC][GT]AC
AccIII 4936 TCCGGA T^CCGGA
BalI 1661 TGGCCA TGG^CCA
BclI 4793 TGATCA T^GATCA
BglII 2482 AGATCT A^GATCT
BseAI 4936 TCCGGA T^CCGGA
Bsp1407I 5373 TGTACA T^GTACA
BspEI 4936 TCCGGA T^CCGGA
BsrDI 1337 CATTGC GCAATG(2/0)
BsrGI 5373 TGTACA T^GTACA
Bst1107I 5216 GTATAC GTA^TAC
BstZ17I 5216 GTATAC GTA^TAC
CpoI 1032 CGGACCG CG^G[AT]CCG
EcoRI 3894 GAATTC G^AATTC
EcoT22I 168 ATGCAT ATGCA^T
FbaI 4793 TGATCA T^GATCA
FseI 731 GGCCGGCC GGCCGG^CC
HincII 5636 GTTAAC GT[CT]^[GA]AC
HindII 5636 GTTAAC GT[CT]^[GA]AC
HindIII 1134 AAGCTT A^AGCTT
HpaI 5636 GTTAAC GTT^AAC
MluNI 1661 TGGCCA TGG^CCA
MroI 4936 TCCGGA T^CCGGA
MscI 1661 TGGCCA TGG^CCA
NcoI 4086 CCATGG C^CATGG
NruI 5471 TCGCGA TCG^CGA
NsiI 168 ATGCAT ATGCA^T
PshAI 1536 GACGGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1191 CGATCG CGAT^CG
RsrII 1032 CGGACCG CG^G[AT]CCG
SspBI 5373 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 4509 GGTACC G^GTACC
5240 GGTACC
AcsI 3894 GAATTC [GA]^AATT[CT]
5454 GAATTT
AflIII 494 ACACGT A^C[GA][CT]GT
1256 ACACGT
Alw44I 508 GTGCAC G^TGCAC
952 GTGCAC
Aor51HI 3997 AGCGCT AGC^GCT
4737 AGCGCT
ApaLI 508 GTGCAC G^TGCAC
952 GTGCAC
ApoI 3894 GAATTC [GA]^AATT[CT]
5454 GAATTT
Asp700I 700 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4035 GAAGACCTTC
Asp718I 4509 GGTACC G^GTACC
5240 GGTACC
AvrII 2603 CCTAGG C^CTAGG
4233 CCTAGG
BcgI 1373 GCACCGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4634 GCATCCCGCTCG
BlnI 2603 CCTAGG C^CTAGG
4233 CCTAGG
BsaI 967 GAGACC GGTCTC(1/5)
4439 GGTCTC
BsmBI 752 GAGACG CGTCTC(1/5)
1534 GAGACG
BsmI 4833 GAATGC GAATGC(1/-1)
3366 GCATTC
BstEII 207 GGTGACC G^GT[ACGT]ACC
315 GGTCACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
315 GGTCACC
BstXI 2280 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4086 CCATGGACATGG
DrdI 8 GACCGCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1465 GACTGGACGGTC
EagI 730 CGGCCG C^GGCCG
734 CGGCCG
EclXI 730 CGGCCG C^GGCCG
734 CGGCCG
Eco47III 3997 AGCGCT AGC^GCT
4737 AGCGCT
Eco52I 730 CGGCCG C^GGCCG
734 CGGCCG
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
315 GGTCACC
KpnI 4509 GGTACC GGTAC^C
5240 GGTACC
PflMI 29 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCAGGGCCTGG
SacI 885 GAGCTC GAGCT^C
4571 GAGCTC
SapI 4786 GAAGAGC GCTCTTC(1/4)
5333 GAAGAGC
SexAI 2194 ACCAGGT A^CC[AT]GGT
2636 ACCAGGT
SfiI 66 GGCCCCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
798 GGCCCTCCGGGCC
SgrAI 1374 CACCGGCG C[GA]^CCGG[CT]G
1436 CGCCGGCG
SmaI 2454 CCCGGG CCC^GGG
2911 CCCGGG
SphI 170 GCATGC GCATG^C
410 GCATGC
Van91I 29 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
447 CCAGGGCCTGG
XbaI 4051 TCTAGA T^CTAGA
4989 TCTAGA
XmaCI 2454 CCCGGG C^CCGGG
2911 CCCGGG
XmaI 2454 CCCGGG C^CCGGG
2911 CCCGGG
XmnI 700 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4035 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI ApaI
AspEI AspHI AvaI AvaII AviII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PaeR7I PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoI XhoII