##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AcsI AflIII Alw44I
ApaLI ApoI AseI AsnI Asp718I
AspI BamHI BciVI BclI BlpI
Bpu1102I BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraI
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MunI NheI NotI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 2783 GACGTC GACGT^C
AccI 218 GTCTAC GT^[AC][GT]AC
AccIII 3431 TCCGGA T^CCGGA
AgeI 2853 ACCGGT A^CCGGT
AhdI 4553 GACGCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 3766 GGCGCGCC GG^CGCGCC
Asp700I 2893 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4553 GACGCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1231 CCTAGG C^CTAGG
BalI 5398 TGGCCA TGG^CCA
BlnI 1231 CCTAGG C^CTAGG
BseAI 3431 TCCGGA T^CCGGA
BsmBI 5163 GAGACG CGTCTC(1/5)
BsmI 499 GCATTC GAATGC(1/-1)
BspEI 3431 TCCGGA T^CCGGA
BsrBI 137 GAGCGG CCGCTC(-3/-3)
BsrDI 1173 CATTGC GCAATG(2/0)
Eam1105I 4553 GACGCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
MluNI 5398 TGGCCA TGG^CCA
MroI 3431 TCCGGA T^CCGGA
MscI 5398 TGGCCA TGG^CCA
NdeI 1560 CATATG CA^TATG
NruI 186 TCGCGA TCG^CGA
NspI 1894 ACATGC [GA]CATG^[CT]
PinAI 2853 ACCGGT A^CCGGT
SexAI 2449 ACCTGGT A^CC[AT]GGT
XmnI 2893 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 950 CTTAAG C^TTAAG
1672 CTTAAG
BbrPI 17 CACGTG CAC^GTG
2301 CACGTG
BfrI 950 CTTAAG C^TTAAG
1672 CTTAAG
BglII 638 AGATCT A^GATCT
3470 AGATCT
BsaWI 2853 ACCGGT [AT]^CCGG[AT]
3431 TCCGGA
BssSI 1944 CACGAG CACGAG(-5/-1)
3492 CACGAG
BstEII 1243 GGTGACC G^GT[ACGT]ACC
5548 GGTCACC
BstPI 1243 GGTGACC G^GT[ACGT]ACC
5548 GGTCACC
DrdI 1492 GACTTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3503 GACCTCTGAGTC
EagI 3852 CGGCCG C^GGCCG
4263 CGGCCG
EclXI 3852 CGGCCG C^GGCCG
4263 CGGCCG
Eco52I 3852 CGGCCG C^GGCCG
4263 CGGCCG
EcoO65I 1243 GGTGACC G^GT[ACGT]ACC
5548 GGTCACC
FseI 1757 GGCCGGCC GGCCGG^CC
4264 GGCCGGCC
KspI 4260 CCGCGG CCGC^GG
4312 CCGCGG
NcoI 1714 CCATGG C^CATGG
4320 CCATGG
PmaCI 17 CACGTG CAC^GTG
2301 CACGTG
PmlI 17 CACGTG CAC^GTG
2301 CACGTG
PshAI 4128 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4374 GACGGGGGTC
SacII 4260 CCGCGG CCGC^GG
4312 CCGCGG
SapI 1866 GAAGAGC GCTCTTC(1/4)
4399 GAAGAGC
SfiI 1752 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4456 GGCCAGCACGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII AviII BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI