##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AccIII AclI AgeI AhdI AseI
AsnI AspEI AviII BamHI BclI
BseAI BsiWI BsmBI BspDI BspEI
BstBI ClaI CpoI DrdI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SexAI
SfuI SnaBI SpeI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3854 GACGTC GACGT^C
Acc65I 759 GGTACC G^GTACC
AflII 461 CTTAAG C^TTAAG
AflIII 5456 ACATGT A^C[GA][CT]GT
Aor51HI 3042 AGCGCT AGC^GCT
Asp700I 5489 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 759 GGTACC G^GTACC
BbrPI 4504 CACGTG CAC^GTG
BciVI 1063 GTATCC GTATCC(6/5)
BfrI 461 CTTAAG C^TTAAG
BglII 328 AGATCT A^GATCT
BsaBI 1213 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3372 CGGCCG CG[GA][CT]^CG
BsmI 1525 GAATGC GAATGC(1/-1)
BspHI 5033 TCATGA T^CATGA
BspLU11I 5456 ACATGT A^CATGT
Bst1107I 2234 GTATAC GTA^TAC
BstAPI 2332 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2983 GGTGACC G^GT[ACGT]ACC
BstPI 2983 GGTGACC G^GT[ACGT]ACC
BstZ17I 2234 GTATAC GTA^TAC
DraI 5060 TTTAAA TTT^AAA
EagI 3372 CGGCCG C^GGCCG
EclXI 3372 CGGCCG C^GGCCG
Eco47III 3042 AGCGCT AGC^GCT
Eco52I 3372 CGGCCG C^GGCCG
EcoO65I 2983 GGTGACC G^GT[ACGT]ACC
HindIII 5330 AAGCTT A^AGCTT
KpnI 759 GGTACC GGTAC^C
MamI 1213 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 909 CCATGG C^CATGG
NdeI 5351 CATATG CA^TATG
PmaCI 4504 CACGTG CAC^GTG
PmlI 4504 CACGTG CAC^GTG
RcaI 5033 TCATGA T^CATGA
SalI 1346 GTCGAC G^TCGAC
SbfI 5025 CCTGCAGG CCTGCA^GG
ScaI 4244 AGTACT AGT^ACT
SgrAI 1118 CGCCGGTG C[GA]^CCGG[CT]G
SphI 589 GCATGC GCATG^C
Sse8387I 5025 CCTGCAGG CCTGCA^GG
TaqI 1347 TCGA T^CGA
TthHB8I 1347 TCGA T^CGA
XbaI 4519 TCTAGA T^CTAGA
XmnI 5489 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1331 AAATTT [GA]^AATT[CT]
5528 AAATTT
AocI 259 CCTGAGG CC^T[ACGT]AGG
882 CCTCAGG
ApoI 1331 AAATTT [GA]^AATT[CT]
5528 AAATTT
AscI 14 GGCGCGCC GG^CGCGCC
3156 GGCGCGCC
AspI 856 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACGACGTC
AvrII 4382 CCTAGG C^CTAGG
5183 CCTAGG
BcgI 982 CGAGCGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1452 GCAGCCGCATCG
BlnI 4382 CCTAGG C^CTAGG
5183 CCTAGG
BlpI 76 GCTGAGC GC^T[ACGT]AGC
2179 GCTGAGC
Bpu1102I 76 GCTGAGC GC^T[ACGT]AGC
2179 GCTGAGC
BsaAI 1574 TACGTG [CT]AC^GT[GA]
4504 CACGTG
BsaI 365 GAGACC GGTCTC(1/5)
956 GAGACC
Bsp1407I 1197 TGTACA T^GTACA
1263 TGTACA
BsrDI 216 GCAATG GCAATG(2/0)
2157 GCAATG
BsrGI 1197 TGTACA T^GTACA
1263 TGTACA
BssSI 4414 CACGAG CACGAG(-5/-1)
3151 CTCGTG
Bsu36I 259 CCTGAGG CC^T[ACGT]AGG
882 CCTCAGG
CelII 76 GCTGAGC GC^T[ACGT]AGC
2179 GCTGAGC
DraIII 2225 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4455 CACAAGGTG
Eco81I 259 CCTGAGG CC^T[ACGT]AGG
882 CCTCAGG
HincII 1346 GTCGAC GT[CT]^[GA]AC
2630 GTCAAC
HindII 1346 GTCGAC GT[CT]^[GA]AC
2630 GTCAAC
KspI 1000 CCGCGG CCGC^GG
3183 CCGCGG
NheI 1280 GCTAGC G^CTAGC
4421 GCTAGC
PflFI 856 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACGACGTC
SacI 2504 GAGCTC GAGCT^C
4989 GAGCTC
SacII 1000 CCGCGG CCGC^GG
3183 CCGCGG
SapI 3913 GAAGAGC GCTCTTC(1/4)
4873 GAAGAGC
SfiI 3007 GGCCATGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3716 GGCCGGGCAGGCC
SspBI 1197 TGTACA T^GTACA
1263 TGTACA
Tsp509I 1332 AATT ^AATT
5529 AATT
Tth111I 856 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3851 GACGACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I TspRI
Van91I XcmI XhoII XmaCI XmaI