##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AclI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I AspEI AspI AviII
AvrII BbeI BbrPI BciVI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI BspDI BspHI BssHII BstBI
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FseI FspI HpaI KasI
KspI MluI NarI NdeI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1355 GACGTC GACGT^C
Acc65I 17 GGTACC G^GTACC
AccIII 908 TCCGGA T^CCGGA
AcsI 585 GAATTC [GA]^AATT[CT]
AflII 2355 CTTAAG C^TTAAG
AgeI 20 ACCGGT A^CCGGT
ApoI 585 GAATTC [GA]^AATT[CT]
AseI 3361 ATTAAT AT^TAAT
AsnI 3361 ATTAAT AT^TAAT
Asp718I 17 GGTACC G^GTACC
BamHI 911 GGATCC G^GATCC
BclI 1128 TGATCA T^GATCA
BfrI 2355 CTTAAG C^TTAAG
BglII 1398 AGATCT A^GATCT
BsaI 2865 GAGACC GGTCTC(1/5)
BseAI 908 TCCGGA T^CCGGA
BsmI 395 GAATGC GAATGC(1/-1)
Bsp1407I 1080 TGTACA T^GTACA
BspEI 908 TCCGGA T^CCGGA
BsrDI 3431 GCAATG GCAATG(2/0)
BsrGI 1080 TGTACA T^GTACA
Bst1107I 1388 GTATAC GTA^TAC
BstAPI 4482 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1388 GTATAC GTA^TAC
DraI 3354 TTTAAA TTT^AAA
EcoRI 585 GAATTC G^AATTC
FbaI 1128 TGATCA T^GATCA
HaeII 903 GGCGCT [GA]GCGC^[CT]
HgaI 698 GCGTC GACGC(5/10)
HincII 2 GTCGAC GT[CT]^[GA]AC
HindII 2 GTCGAC GT[CT]^[GA]AC
HindIII 3929 AAGCTT A^AGCTT
KpnI 17 GGTACC GGTAC^C
MfeI 3558 CAATTG C^AATTG
MroI 908 TCCGGA T^CCGGA
MunI 3558 CAATTG C^AATTG
NheI 4585 GCTAGC G^CTAGC
PaeR7I 1705 CTCGAG C^TCGAG
PinAI 20 ACCGGT A^CCGGT
PshBI 3361 ATTAAT AT^TAAT
SalI 2 GTCGAC G^TCGAC
SexAI 4503 ACCTGGT A^CC[AT]GGT
SmaI 2312 CCCGGG CCC^GGG
SspBI 1080 TGTACA T^GTACA
SspI 3358 AATATT AAT^ATT
XhoI 1705 CTCGAG C^TCGAG
XmaCI 2312 CCCGGG C^CCGGG
XmaI 2312 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1162 CGCG CG^CG
3339 CGCG
AcyI 697 GGCGTC G[GA]^CG[CT]C
1355 GACGTC
ApaI 257 GGGCCC GGGCC^C
4050 GGGCCC
BalI 848 TGGCCA TGG^CCA
1093 TGGCCA
BsaBI 1123 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2934 GATGCTTATC
BsaHI 697 GGCGTC G[GA]^CG[CT]C
1355 GACGTC
BsaWI 20 ACCGGT [AT]^CCGG[AT]
908 TCCGGA
BspLU11I 143 ACATGT A^CATGT
2987 ACATGT
BstUI 1162 CGCG CG^CG
3339 CGCG
BstXI 1713 CCATTTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3308 CCAAAGTGCTGG
BstYI 911 GGATCC [GA]^GATC[CT]
1398 AGATCT
DraIII 240 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4502 CACCTGGTG
EcoNI 74 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2453 CCTGGAAGAGG
Hin1I 697 GGCGTC G[GA]^CG[CT]C
1355 GACGTC
MamI 1123 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2934 GATGCTTATC
MflI 911 GGATCC [GA]^GATC[CT]
1398 AGATCT
MluNI 848 TGGCCA TGG^CCA
1093 TGGCCA
MscI 848 TGGCCA TGG^CCA
1093 TGGCCA
MvnI 1162 CGCG CG^CG
3339 CGCG
NaeI 628 GCCGGC GCC^GGC
4194 GCCGGC
NgoMIV 628 GCCGGC G^CCGGC
4194 GCCGGC
PstI 1748 CTGCAG CTGCA^G
4489 CTGCAG
SapI 874 GAAGAGC GCTCTTC(1/4)
2543 GAAGAGC
SphI 1730 GCATGC GCATG^C
3194 GCATGC
XhoII 911 GGATCC [GA]^GATC[CT]
1398 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AciI AfaI AflIII AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI