##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI ApoI AscI Asp718I
AspI AviII AvrII BbeI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
DraIII EcoRI EcoRV FseI FspI
HindIII HpaI KasI KpnI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tsp509I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1367 GACGTC GACGT^C
AhdI 4253 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1082 AGCGCT AGC^GCT
AseI 1609 ATTAAT AT^TAAT
AsnI 1609 ATTAAT AT^TAAT
AspEI 4253 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 4123 CACGTG CAC^GTG
BciVI 1826 GTATCC GTATCC(6/5)
BclI 3186 TGATCA T^GATCA
BglII 4304 AGATCT A^GATCT
BsaBI 1267 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 657 TGTACA T^GTACA
BspLU11I 6 ACATGT A^CATGT
BsrGI 657 TGTACA T^GTACA
CpoI 1463 CGGACCG CG^G[AT]CCG
DraI 3572 TTTAAA TTT^AAA
DrdI 3892 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4253 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1082 AGCGCT AGC^GCT
EcoT22I 3309 ATGCAT ATGCA^T
FbaI 3186 TGATCA T^GATCA
HaeII 1082 AGCGCT [GA]GCGC^[CT]
HincII 671 GTTGAC GT[CT]^[GA]AC
HindII 671 GTTGAC GT[CT]^[GA]AC
MamI 1267 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2563 GCCGGC GCC^GGC
NgoMIV 2563 GCCGGC G^CCGGC
NsiI 3309 ATGCAT ATGCA^T
PaeR7I 3249 CTCGAG C^TCGAG
PmaCI 4123 CACGTG CAC^GTG
PmlI 4123 CACGTG CAC^GTG
PshBI 1609 ATTAAT AT^TAAT
RsrII 1463 CGGACCG CG^G[AT]CCG
SapI 3154 GCTCTTC GCTCTTC(1/4)
ScaI 2634 AGTACT AGT^ACT
SexAI 3135 ACCAGGT A^CC[AT]GGT
SfiI 1888 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 657 TGTACA T^GTACA
SspI 1606 AATATT AAT^ATT
XhoI 3249 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 650 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3944 CAGCACCTG
BamHI 1227 GGATCC G^GATCC
2306 GGATCC
BcgI 1768 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3414 CGAGCCTCGTGC
BsaAI 635 TACGTG [CT]AC^GT[GA]
4123 CACGTG
BsmBI 3512 CGTCTC CGTCTC(1/5)
2001 GAGACG
BsmI 2909 GCATTC GAATGC(1/-1)
3451 GCATTC
BspHI 582 TCATGA T^CATGA
3183 TCATGA
BstEII 418 GGTGACC G^GT[ACGT]ACC
1006 GGTCACC
BstPI 418 GGTGACC G^GT[ACGT]ACC
1006 GGTCACC
EcoO65I 418 GGTGACC G^GT[ACGT]ACC
1006 GGTCACC
FauI 2975 CCCGC CCCGC(4/6)
1363 GCGGG
KspI 853 CCGCGG CCGC^GG
2976 CCGCGG
MseI 1610 TTAA T^TAA
3573 TTAA
NspI 6 ACATGT [GA]CATG^[CT]
1711 GCATGT
PflMI 3943 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3966 CCAAGCCCTGG
RcaI 582 TCATGA T^CATGA
3183 TCATGA
SacII 853 CCGCGG CCGC^GG
2976 CCGCGG
TfiI 915 GAATC G^A[AT]TC
3128 GATTC
Tru9I 1610 TTAA T^TAA
3573 TTAA
Van91I 3943 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3966 CCAAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AocI ApaI
ApaLI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PshAI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI XmnI