##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AscI AseI AsnI Asp718I AviII
BclI BfrI BsaBI BsiWI BspDI
BspLU11I BsrDI Bst1107I BstBI BstZ17I
ClaI CpoI DraIII EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI TfiI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 3248 GACGTC GACGT^C
AccIII 1032 TCCGGA T^CCGGA
AflIII 1242 ACGTGT A^C[GA][CT]GT
AgeI 2223 ACCGGT A^CCGGT
AhdI 32 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2041 CCTCAGG CC^T[ACGT]AGG
Asp700I 1240 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 32 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1775 GGATCC G^GATCC
BbrPI 2033 CACGTG CAC^GTG
BciVI 2474 GGATAC GTATCC(6/5)
BglII 2748 AGATCT A^GATCT
BseAI 1032 TCCGGA T^CCGGA
BspEI 1032 TCCGGA T^CCGGA
BspHI 2061 TCATGA T^CATGA
BstEII 3046 GGTCACC G^GT[ACGT]ACC
BstPI 3046 GGTCACC G^GT[ACGT]ACC
Bsu36I 2041 CCTCAGG CC^T[ACGT]AGG
DraI 4355 TTTAAA TTT^AAA
Eam1105I 32 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2041 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3046 GGTCACC G^GT[ACGT]ACC
HincII 1889 GTCAAC GT[CT]^[GA]AC
HindII 1889 GTCAAC GT[CT]^[GA]AC
MroI 1032 TCCGGA T^CCGGA
MseI 4356 TTAA T^TAA
NdeI 4338 CATATG CA^TATG
PflFI 1730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2223 ACCGGT A^CCGGT
PmaCI 2033 CACGTG CAC^GTG
PmlI 2033 CACGTG CAC^GTG
PshAI 857 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2061 TCATGA T^CATGA
SacI 1904 GAGCTC GAGCT^C
SbfI 3163 CCTGCAGG CCTGCA^GG
ScaI 2652 AGTACT AGT^ACT
SmlI 632 CTCAAG C^T[CT][GA]AG
Sse8387I 3163 CCTGCAGG CCTGCA^GG
Tru9I 4356 TTAA T^TAA
Tth111I 1730 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1240 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 947 GTCTAC GT^[AC][GT]AC
1202 GTCTAC
Aor51HI 1320 AGCGCT AGC^GCT
2055 AGCGCT
AvrII 3848 CCTAGG C^CTAGG
3905 CCTAGG
BbvCI 2371 CCTCAGC CCTCAGC(-5/-2)
260 GCTGAGG
BglI 774 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3236 GCCGTCATGGC
BlnI 3848 CCTAGG C^CTAGG
3905 CCTAGG
BsaAI 311 TACGTG [CT]AC^GT[GA]
2033 CACGTG
BsaWI 1032 TCCGGA [AT]^CCGG[AT]
2223 ACCGGT
BsmI 877 GAATGC GAATGC(1/-1)
2638 GAATGC
BssSI 482 CACGAG CACGAG(-5/-1)
2078 CTCGTG
EarI 740 CTCTTC CTCTTC(1/4)
1066 GAAGAG
Eco47III 1320 AGCGCT AGC^GCT
2055 AGCGCT
EcoNI 82 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4260 CCTCTCCCAGG
Ksp632I 740 CTCTTC CTCTTC(1/4)
1066 GAAGAG
NcoI 1083 CCATGG C^CATGG
1098 CCATGG
PflMI 96 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCAGCGGGTGG
Tsp509I 1519 AATT ^AATT
4210 AATT
Van91I 96 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCAGCGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
SspBI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI