##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI BcgI BciVI
BclI BfrI BsiWI BsmI Bsp1407I
BspHI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MfeI MluI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1362 GACGTC GACGT^C
AflIII 2116 ACATGT A^C[GA][CT]GT
AocI 404 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2627 AGCGCT AGC^GCT
AspI 1020 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4470 CCTAGG C^CTAGG
BalI 2692 TGGCCA TGG^CCA
BbrPI 3936 CACGTG CAC^GTG
BbvCI 1257 GCTGAGG CCTCAGC(-5/-2)
BfaI 4471 CTAG C^TAG
BlnI 4470 CCTAGG C^CTAGG
BsaAI 3936 CACGTG [CT]AC^GT[GA]
BsaI 327 GAGACC GGTCTC(1/5)
BspLU11I 2116 ACATGT A^CATGT
BsrDI 4301 CATTGC GCAATG(2/0)
BstEII 1479 GGTCACC G^GT[ACGT]ACC
BstPI 1479 GGTCACC G^GT[ACGT]ACC
Bsu36I 404 CCTTAGG CC^T[ACGT]AGG
DraIII 2965 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2738 GACGGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1408 CGGCCG C^GGCCG
EclXI 1408 CGGCCG C^GGCCG
Eco47III 2627 AGCGCT AGC^GCT
Eco52I 1408 CGGCCG C^GGCCG
Eco81I 404 CCTTAGG CC^T[ACGT]AGG
EcoO65I 1479 GGTCACC G^GT[ACGT]ACC
MaeI 4471 CTAG C^TAG
MluNI 2692 TGGCCA TGG^CCA
MscI 2692 TGGCCA TGG^CCA
PaeR7I 1887 CTCGAG C^TCGAG
PflFI 1020 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3936 CACGTG CAC^GTG
PmlI 3936 CACGTG CAC^GTG
ScaI 418 AGTACT AGT^ACT
SmaI 2128 CCCGGG CCC^GGG
SphI 4438 GCATGC GCATG^C
SspI 3826 AATATT AAT^ATT
Tth111I 1020 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1887 CTCGAG C^TCGAG
XmaCI 2128 CCCGGG C^CCGGG
XmaI 2128 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3636 GTCGAC GT^[AC][GT]AC
4005 GTCGAC
AccIII 1445 TCCGGA T^CCGGA
2767 TCCGGA
Asp700I 509 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1238 GAAGGAGTTC
AvaI 1887 CTCGAG C^[CT]CG[GA]G
2128 CCCGGG
AviII 874 TGCGCA TGC^GCA
1780 TGCGCA
BamHI 2536 GGATCC G^GATCC
3856 GGATCC
BbeI 1179 GGCGCC GGCGC^C
1927 GGCGCC
BsaBI 68 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2267 GATCGAGATC
BseAI 1445 TCCGGA T^CCGGA
2767 TCCGGA
BsiEI 1408 CGGCCG CG[GA][CT]^CG
3631 CGGTCG
BsoBI 1887 CTCGAG C^[CT]CG[GA]G
2128 CCCGGG
BspDI 2862 ATCGAT AT^CGAT
3069 ATCGAT
BspEI 1445 TCCGGA T^CCGGA
2767 TCCGGA
BsrBI 2912 CCGCTC CCGCTC(-3/-3)
1707 GAGCGG
BssSI 4357 CACGAG CACGAG(-5/-1)
2715 CTCGTG
ClaI 2862 ATCGAT AT^CGAT
3069 ATCGAT
FspI 874 TGCGCA TGC^GCA
1780 TGCGCA
KasI 1179 GGCGCC G^GCGCC
1927 GGCGCC
MamI 68 GATTCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2267 GATCGAGATC
MroI 1445 TCCGGA T^CCGGA
2767 TCCGGA
MseI 2230 TTAA T^TAA
3280 TTAA
NarI 1179 GGCGCC GG^CGCC
1927 GGCGCC
PshAI 1473 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3629 GACGGTCGTC
SalI 3636 GTCGAC G^TCGAC
4005 GTCGAC
SfoI 1179 GGCGCC GGC^GCC
1927 GGCGCC
StuI 3184 AGGCCT AGG^CCT
4473 AGGCCT
Tru9I 2230 TTAA T^TAA
3280 TTAA
XmnI 509 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1238 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII