##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AflIII AhdI
Aor51HI AseI AsnI AspEI BbrPI
BcgI BciVI BglII BsaAI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BstBI
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KspI MamI MluI MroI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1248 GGTACC G^GTACC
AgeI 1728 ACCGGT A^CCGGT
AscI 219 GGCGCGCC GG^CGCGCC
Asp718I 1248 GGTACC G^GTACC
AspI 2403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3655 TGCGCA TGC^GCA
BamHI 236 GGATCC G^GATCC
BbeI 493 GGCGCC GGCGC^C
BclI 4988 TGATCA T^GATCA
BsaWI 1728 ACCGGT [AT]^CCGG[AT]
BsmBI 2539 GAGACG CGTCTC(1/5)
BspHI 1335 TCATGA T^CATGA
BsrBI 3424 GAGCGG CCGCTC(-3/-3)
BssHII 220 GCGCGC G^CGCGC
Bst1107I 2280 GTATAC GTA^TAC
BstZ17I 2280 GTATAC GTA^TAC
DraIII 2012 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4274 GACTGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 4988 TGATCA T^GATCA
FspI 3655 TGCGCA TGC^GCA
KasI 493 GGCGCC G^GCGCC
KpnI 1248 GGTACC GGTAC^C
MfeI 2080 CAATTG C^AATTG
MunI 2080 CAATTG C^AATTG
NarI 493 GGCGCC GG^CGCC
NcoI 4397 CCATGG C^CATGG
NspI 775 GCATGT [GA]CATG^[CT]
PflFI 2403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1728 ACCGGT A^CCGGT
PshAI 310 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1335 TCATGA T^CATGA
SapI 2446 GCTCTTC GCTCTTC(1/4)
SbfI 854 CCTGCAGG CCTGCA^GG
ScaI 640 AGTACT AGT^ACT
SfoI 493 GGCGCC GGC^GCC
SmaI 770 CCCGGG CCC^GGG
Sse8387I 854 CCTGCAGG CCTGCA^GG
Tth111I 2403 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 770 CCCGGG C^CCGGG
XmaI 770 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 221 CGCG CG^CG
2136 CGCG
AcyI 493 GGCGCC G[GA]^CG[CT]C
3229 GGCGTC
AflII 891 CTTAAG C^TTAAG
1572 CTTAAG
Alw44I 475 GTGCAC G^TGCAC
1711 GTGCAC
ApaLI 475 GTGCAC G^TGCAC
1711 GTGCAC
Asp700I 596 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1791 GAACATTTTC
AvaI 770 CCCGGG C^[CT]CG[GA]G
3668 CTCGGG
BfrI 891 CTTAAG C^TTAAG
1572 CTTAAG
BlpI 794 GCTTAGC GC^T[ACGT]AGC
3035 GCTTAGC
Bpu1102I 794 GCTTAGC GC^T[ACGT]AGC
3035 GCTTAGC
BsaHI 493 GGCGCC G[GA]^CG[CT]C
3229 GGCGTC
BsaI 2639 GAGACC GGTCTC(1/5)
4748 GAGACC
BsoBI 770 CCCGGG C^[CT]CG[GA]G
3668 CTCGGG
BssSI 900 CACGAG CACGAG(-5/-1)
4732 CTCGTG
BstEII 402 GGTGACC G^GT[ACGT]ACC
3835 GGTGACC
BstPI 402 GGTGACC G^GT[ACGT]ACC
3835 GGTGACC
BstUI 221 CGCG CG^CG
2136 CGCG
BstYI 236 GGATCC [GA]^GATC[CT]
2321 GGATCT
CelII 794 GCTTAGC GC^T[ACGT]AGC
3035 GCTTAGC
DsaI 607 CCACGG C^C[GA][CT]GG
4397 CCATGG
EcoO65I 402 GGTGACC G^GT[ACGT]ACC
3835 GGTGACC
EcoRI 1339 GAATTC G^AATTC
3802 GAATTC
Hin1I 493 GGCGCC G[GA]^CG[CT]C
3229 GGCGTC
MflI 236 GGATCC [GA]^GATC[CT]
2321 GGATCT
MvnI 221 CGCG CG^CG
2136 CGCG
NaeI 664 GCCGGC GCC^GGC
861 GCCGGC
NgoMIV 664 GCCGGC G^CCGGC
861 GCCGGC
NheI 2442 GCTAGC G^CTAGC
2961 GCTAGC
PflMI 192 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCAGGATGTGG
SspI 1990 AATATT AAT^ATT
2477 AATATT
Van91I 192 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCAGGATGTGG
XbaI 3134 TCTAGA T^CTAGA
3420 TCTAGA
XhoII 236 GGATCC [GA]^GATC[CT]
2321 GGATCT
XmnI 596 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1791 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII AvrII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI