##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbvCI BlnI BsiWI
BsmI BspDI BspLU11I BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRV EcoT22I FseI FspI HindIII
KspI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 728 GGTACC G^GTACC
AccIII 1345 TCCGGA T^CCGGA
AcsI 2589 GAATTC [GA]^AATT[CT]
AflII 3034 CTTAAG C^TTAAG
Alw44I 2829 GTGCAC G^TGCAC
Aor51HI 889 AGCGCT AGC^GCT
ApaLI 2829 GTGCAC G^TGCAC
ApoI 2589 GAATTC [GA]^AATT[CT]
Asp718I 728 GGTACC G^GTACC
BamHI 3636 GGATCC G^GATCC
BbrPI 22 CACGTG CAC^GTG
BbsI 972 GAAGAC GAAGAC(2/6)
BcgI 3776 GCACCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3411 GTATCC GTATCC(6/5)
BclI 1871 TGATCA T^GATCA
BfrI 3034 CTTAAG C^TTAAG
BpuAI 972 GAAGAC GAAGAC(2/6)
BsaBI 1233 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1345 TCCGGA [AT]^CCGG[AT]
BseAI 1345 TCCGGA T^CCGGA
BspEI 1345 TCCGGA T^CCGGA
BssHII 7 GCGCGC G^CGCGC
BstAPI 552 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1792 GGTCACC G^GT[ACGT]ACC
BstPI 1792 GGTCACC G^GT[ACGT]ACC
DraI 3959 TTTAAA TTT^AAA
DraIII 165 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 889 AGCGCT AGC^GCT
EcoO65I 1792 GGTCACC G^GT[ACGT]ACC
EcoRI 2589 GAATTC G^AATTC
FbaI 1871 TGATCA T^GATCA
HincII 2052 GTTAAC GT[CT]^[GA]AC
HindII 2052 GTTAAC GT[CT]^[GA]AC
HpaI 2052 GTTAAC GTT^AAC
KpnI 728 GGTACC GGTAC^C
MamI 1233 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1345 TCCGGA T^CCGGA
PaeR7I 3508 CTCGAG C^TCGAG
PmaCI 22 CACGTG CAC^GTG
PmlI 22 CACGTG CAC^GTG
SacI 2871 GAGCTC GAGCT^C
SphI 248 GCATGC GCATG^C
SwaI 3958 ATTTAAAT ATTT^AAAT
XhoI 3508 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 21 ACACGT A^C[GA][CT]GT
719 ACGTGT
ApaI 3689 GGGCCC GGGCC^C
3793 GGGCCC
AspI 1617 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2967 GACTTGGTC
BglII 1265 AGATCT A^GATCT
2532 AGATCT
BsaAI 22 CACGTG [CT]AC^GT[GA]
718 TACGTG
Bsp1407I 722 TGTACA T^GTACA
3906 TGTACA
BsrBI 240 CCGCTC CCGCTC(-3/-3)
3619 CCGCTC
BsrGI 722 TGTACA T^GTACA
3906 TGTACA
BssSI 1503 CACGAG CACGAG(-5/-1)
2991 CACGAG
DrdI 1784 GACAGAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACCTCAGAGTC
EagI 431 CGGCCG C^GGCCG
1302 CGGCCG
EclXI 431 CGGCCG C^GGCCG
1302 CGGCCG
Eco52I 431 CGGCCG C^GGCCG
1302 CGGCCG
NspI 220 ACATGC [GA]CATG^[CT]
248 GCATGC
PflFI 1617 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2967 GACTTGGTC
PflMI 2060 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2511 CCATTTTCTGG
SapI 1340 GCTCTTC GCTCTTC(1/4)
1600 GCTCTTC
SspBI 722 TGTACA T^GTACA
3906 TGTACA
StuI 1162 AGGCCT AGG^CCT
3743 AGGCCT
Tsp509I 927 AATT ^AATT
2590 AATT
Tth111I 1617 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2967 GACTTGGTC
Van91I 2060 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2511 CCATTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI