##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I BamHI BbeI
BbrPI BbsI BciVI BclI BfrI
BpuAI BsaAI BsaBI BsaHI BsiWI
BsmBI Bsp1407I BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CpoI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FseI
Hin1I HindIII KasI KpnI MamI
MfeI MluI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SalI SapI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1863 GTAC GT^AC
AflIII 252 ACACGT A^C[GA][CT]GT
AhdI 2757 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 441 GGATC GGATC(4/5)
AspEI 2757 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 43 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1283 TGCGCA TGC^GCA
AvrII 3853 CCTAGG C^CTAGG
BglII 186 AGATCT A^GATCT
BlnI 3853 CCTAGG C^CTAGG
BsmI 3867 GCATTC GAATGC(1/-1)
BspDI 443 ATCGAT AT^CGAT
BstYI 186 AGATCT [GA]^GATC[CT]
ClaI 443 ATCGAT AT^CGAT
Eam1105I 2757 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4142 GAATTC G^AATTC
FspI 1283 TGCGCA TGC^GCA
HpaI 1856 GTTAAC GTT^AAC
KspI 347 CCGCGG CCGC^GG
MflI 186 AGATCT [GA]^GATC[CT]
NaeI 3421 GCCGGC GCC^GGC
NgoMIV 3421 GCCGGC G^CCGGC
PflFI 43 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 376 CGATCG CGAT^CG
RsaI 1863 GTAC GT^AC
SacII 347 CCGCGG CCGC^GG
Tth111I 43 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 186 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1973 GTCTAC GT^[AC][GT]AC
4308 GTCTAC
AccIII 2504 TCCGGA T^CCGGA
3035 TCCGGA
Aor51HI 1904 AGCGCT AGC^GCT
3437 AGCGCT
AvaI 926 CTCGGG C^[CT]CG[GA]G
1546 CCCGAG
BsaWI 2504 TCCGGA [AT]^CCGG[AT]
3035 TCCGGA
BseAI 2504 TCCGGA T^CCGGA
3035 TCCGGA
BsiEI 376 CGATCG CG[GA][CT]^CG
2887 CGACCG
BsoBI 926 CTCGGG C^[CT]CG[GA]G
1546 CCCGAG
BspEI 2504 TCCGGA T^CCGGA
3035 TCCGGA
BsrBI 317 GAGCGG CCGCTC(-3/-3)
559 GAGCGG
DraIII 687 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4149 CACCAGGTG
Eco47III 1904 AGCGCT AGC^GCT
3437 AGCGCT
HgaI 238 GACGC GACGC(5/10)
1108 GCGTC
HincII 1134 GTTGAC GT[CT]^[GA]AC
1856 GTTAAC
HindII 1134 GTTGAC GT[CT]^[GA]AC
1856 GTTAAC
MaeII 254 ACGT A^CGT
2439 ACGT
MroI 2504 TCCGGA T^CCGGA
3035 TCCGGA
MslI 289 CATGACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2212 CACCACTGTG
SacI 2742 GAGCTC GAGCT^C
4133 GAGCTC
SbfI 1953 CCTGCAGG CCTGCA^GG
2932 CCTGCAGG
Sse8387I 1953 CCTGCAGG CCTGCA^GG
2932 CCTGCAGG
SspI 2222 AATATT AAT^ATT
3369 AATATT
StuI 1012 AGGCCT AGG^CCT
1888 AGGCCT
TfiI 2508 GAATC G^A[AT]TC
4256 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AluI AlwNI
AocI ApaI ApoI AspHI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI