##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AhdI AscI Asp700I
AspEI AviII AvrII BciVI BclI
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BssSI
BstBI BstEII BstPI ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1269 GACGTC GACGT^C
Acc65I 2044 GGTACC G^GTACC
AflII 4129 CTTAAG C^TTAAG
AgeI 2123 ACCGGT A^CCGGT
Aor51HI 3910 AGCGCT AGC^GCT
ApaI 3735 GGGCCC GGGCC^C
AseI 1715 ATTAAT AT^TAAT
AsnI 1715 ATTAAT AT^TAAT
Asp718I 2044 GGTACC G^GTACC
AspI 654 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1283 GGATCC G^GATCC
BbeI 849 GGCGCC GGCGC^C
BbsI 4491 GAAGAC GAAGAC(2/6)
BbvCI 2209 GCTGAGG CCTCAGC(-5/-2)
BfrI 4129 CTTAAG C^TTAAG
BglII 1671 AGATCT A^GATCT
BpuAI 4491 GAAGAC GAAGAC(2/6)
BsiEI 2125 CGGTCG CG[GA][CT]^CG
Bsp1407I 1190 TGTACA T^GTACA
BsrBI 1966 CCGCTC CCGCTC(-3/-3)
BsrGI 1190 TGTACA T^GTACA
DrdI 520 GACGTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3910 AGCGCT AGC^GCT
HincII 617 GTTAAC GT[CT]^[GA]AC
HindII 617 GTTAAC GT[CT]^[GA]AC
HpaI 617 GTTAAC GTT^AAC
KasI 849 GGCGCC G^GCGCC
KpnI 2044 GGTACC GGTAC^C
NaeI 2426 GCCGGC GCC^GGC
NarI 849 GGCGCC GG^CGCC
NgoMIV 2426 GCCGGC G^CCGGC
PflFI 654 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2123 ACCGGT A^CCGGT
PshBI 1715 ATTAAT AT^TAAT
SbfI 2003 CCTGCAGG CCTGCA^GG
ScaI 349 AGTACT AGT^ACT
SexAI 1631 ACCTGGT A^CC[AT]GGT
SfiI 2098 GGCCAGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 849 GGCGCC GGC^GCC
Sse8387I 2003 CCTGCAGG CCTGCA^GG
SspBI 1190 TGTACA T^GTACA
Tth111I 654 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 647 GTATAC GT^[AC][GT]AC
1049 GTATAC
AcsI 627 GAATTC [GA]^AATT[CT]
1369 GAATTC
AcyI 849 GGCGCC G[GA]^CG[CT]C
1269 GACGTC
ApoI 627 GAATTC [GA]^AATT[CT]
1369 GAATTC
BalI 1733 TGGCCA TGG^CCA
2831 TGGCCA
BcgI 805 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3326 GCAACTGTCTCG
BsaHI 849 GGCGCC G[GA]^CG[CT]C
1269 GACGTC
BsaWI 2123 ACCGGT [AT]^CCGG[AT]
4242 TCCGGT
BsrDI 4136 CATTGC GCAATG(2/0)
756 GCAATG
BsrFI 2123 ACCGGT [GA]^CCGG[CT]
2426 GCCGGC
BssHII 2262 GCGCGC G^CGCGC
2706 GCGCGC
Bst1107I 647 GTATAC GTA^TAC
1049 GTATAC
BstZ17I 647 GTATAC GTA^TAC
1049 GTATAC
Cfr10I 2123 ACCGGT [GA]^CCGG[CT]
2426 GCCGGC
EarI 1975 GAAGAG CTCTTC(1/4)
2776 GAAGAG
EcoRI 627 GAATTC G^AATTC
1369 GAATTC
HgaI 141 GACGC GACGC(5/10)
273 GACGC
Hin1I 849 GGCGCC G[GA]^CG[CT]C
1269 GACGTC
HindIII 1162 AAGCTT A^AGCTT
4186 AAGCTT
Ksp632I 1975 GAAGAG CTCTTC(1/4)
2776 GAAGAG
MluNI 1733 TGGCCA TGG^CCA
2831 TGGCCA
MscI 1733 TGGCCA TGG^CCA
2831 TGGCCA
SmlI 4129 CTTAAG C^T[CT][GA]AG
4538 CTTGAG
SphI 108 GCATGC GCATG^C
470 GCATGC
XcmI 1971 CCAGGAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2489 CCACCGTGATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
AspHI AvaI AvaII BanI BanII
BbrPI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI