##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AclI AcsI AgeI Aor51HI
ApoI AscI AseI AsnI Asp718I
AvrII BamHI BciVI BclI BglII
BlnI BsaBI BsiWI BspDI BspHI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1142 GACGTC GACGT^C
AccI 2457 GTCTAC GT^[AC][GT]AC
AccIII 1882 TCCGGA T^CCGGA
AflII 4311 CTTAAG C^TTAAG
AflIII 583 ACACGT A^C[GA][CT]GT
Asp700I 1814 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 656 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1612 TGCGCA TGC^GCA
BbeI 4140 GGCGCC GGCGC^C
BfrI 4311 CTTAAG C^TTAAG
BsaWI 1882 TCCGGA [AT]^CCGG[AT]
BseAI 1882 TCCGGA T^CCGGA
BsiEI 317 CGGCCG CG[GA][CT]^CG
BsmI 2083 GCATTC GAATGC(1/-1)
BspEI 1882 TCCGGA T^CCGGA
BstEII 905 GGTCACC G^GT[ACGT]ACC
BstPI 905 GGTCACC G^GT[ACGT]ACC
DraIII 2514 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 317 CGGCCG C^GGCCG
EclXI 317 CGGCCG C^GGCCG
Eco52I 317 CGGCCG C^GGCCG
EcoO65I 905 GGTCACC G^GT[ACGT]ACC
FspI 1612 TGCGCA TGC^GCA
KasI 4140 GGCGCC G^GCGCC
MroI 1882 TCCGGA T^CCGGA
NarI 4140 GGCGCC GG^CGCC
PflFI 656 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 921 GAGCTC GAGCT^C
SapI 4422 GAAGAGC GCTCTTC(1/4)
SexAI 1345 ACCTGGT A^CC[AT]GGT
SfoI 4140 GGCGCC GGC^GCC
TaqI 794 TCGA T^CGA
Tsp509I 4383 AATT ^AATT
Tth111I 656 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 794 TCGA T^CGA
XbaI 4336 TCTAGA T^CTAGA
XmnI 1814 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 579 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1248 GACATCGTGTC
AspEI 579 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1248 GACATCGTGTC
BbrPI 1113 CACGTG CAC^GTG
2514 CACGTG
BcgI 761 CGAGGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1688 GCATGAGCTTCG
BglI 3088 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3648 GCCCACATGGC
BsgI 24 CTGCAC GTGCAG(16/14)
4282 CTGCAC
BsmBI 954 GAGACG CGTCTC(1/5)
2439 GAGACG
BstAPI 462 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2192 GCAGGAGCTGC
BstXI 235 CCAAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1459 CCACACTCATGG
Eam1105I 579 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1248 GACATCGTGTC
PmaCI 1113 CACGTG CAC^GTG
2514 CACGTG
PmlI 1113 CACGTG CAC^GTG
2514 CACGTG
SbfI 374 CCTGCAGG CCTGCA^GG
410 CCTGCAGG
SmaI 492 CCCGGG CCC^GGG
2315 CCCGGG
Sse8387I 374 CCTGCAGG CCTGCA^GG
410 CCTGCAGG
StuI 433 AGGCCT AGG^CCT
2934 AGGCCT
XmaCI 492 CCCGGG C^CCGGG
2315 CCCGGG
XmaI 492 CCCGGG C^CCGGG
2315 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I TspRI Van91I XcmI XhoII