##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI Asp700I AspI AviII
AvrII BalI BclI BfrI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRV
EcoT22I FbaI FspI HpaI MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NotI NruI NsiI
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SgrAI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 3569 CGCG CG^CG
AhdI 645 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1383 AGCGCT AGC^GCT
AspEI 645 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1587 GGATCC G^GATCC
BbeI 2566 GGCGCC GGCGC^C
BbrPI 1620 CACGTG CAC^GTG
BcgI 3318 GCAGCAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4065 GTATCC GTATCC(6/5)
BglII 698 AGATCT A^GATCT
BsaAI 1620 CACGTG [CT]AC^GT[GA]
BsgI 147 GTGCAG GTGCAG(16/14)
BsmBI 3783 GAGACG CGTCTC(1/5)
BstAPI 2988 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3581 TTCGAA TT^CGAA
BstEII 2485 GGTGACC G^GT[ACGT]ACC
BstPI 2485 GGTGACC G^GT[ACGT]ACC
BstUI 3569 CGCG CG^CG
Eam1105I 645 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1383 AGCGCT AGC^GCT
EcoO65I 2485 GGTGACC G^GT[ACGT]ACC
EcoRI 1116 GAATTC G^AATTC
FseI 1569 GGCCGGCC GGCCGG^CC
HindIII 1445 AAGCTT A^AGCTT
KasI 2566 GGCGCC G^GCGCC
KspI 3568 CCGCGG CCGC^GG
MvnI 3569 CGCG CG^CG
NaeI 1570 GCCGGC GCC^GGC
NarI 2566 GGCGCC GG^CGCC
NcoI 1262 CCATGG C^CATGG
NgoMIV 1570 GCCGGC G^CCGGC
NheI 1677 GCTAGC G^CTAGC
NspI 166 GCATGT [GA]CATG^[CT]
NspV 3581 TTCGAA TT^CGAA
PaeR7I 2062 CTCGAG C^TCGAG
PmaCI 1620 CACGTG CAC^GTG
PmlI 1620 CACGTG CAC^GTG
SacII 3568 CCGCGG CCGC^GG
SfiI 2952 GGCCTTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2566 GGCGCC GGC^GCC
SfuI 3581 TTCGAA TT^CGAA
SmaI 481 CCCGGG CCC^GGG
SpeI 3922 ACTAGT A^CTAGT
XhoI 2062 CTCGAG C^TCGAG
XmaCI 481 CCCGGG C^CCGGG
XmaI 481 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 1431 GACGCC G[GA]^CG[CT]C
2566 GGCGCC
ApaI 1727 GGGCCC GGGCC^C
3274 GGGCCC
BlpI 633 GCTGAGC GC^T[ACGT]AGC
3517 GCTCAGC
Bpu1102I 633 GCTGAGC GC^T[ACGT]AGC
3517 GCTCAGC
BsaHI 1431 GACGCC G[GA]^CG[CT]C
2566 GGCGCC
BsrFI 1570 GCCGGC [GA]^CCGG[CT]
2138 ACCGGC
CelII 633 GCTGAGC GC^T[ACGT]AGC
3517 GCTCAGC
Cfr10I 1570 GCCGGC [GA]^CCGG[CT]
2138 ACCGGC
DraIII 598 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1961 CACTTTGTG
DrdI 2293 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3422 GACACGGATGTC
HaeII 1383 AGCGCT [GA]GCGC^[CT]
2566 GGCGCC
HgaI 1431 GACGC GACGC(5/10)
3844 GCGTC
Hin1I 1431 GACGCC G[GA]^CG[CT]C
2566 GGCGCC
HincII 199 GTCAAC GT[CT]^[GA]AC
3456 GTCAAC
HindII 199 GTCAAC GT[CT]^[GA]AC
3456 GTCAAC
MseI 2214 TTAA T^TAA
2328 TTAA
MslI 3758 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3857 CACATTTATG
PshAI 313 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
816 GACCCAGGTC
SacI 1466 GAGCTC GAGCT^C
2582 GAGCTC
Tru9I 2214 TTAA T^TAA
2328 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp718I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII