##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI ApaI ApoI AscI AseI
AsnI AspEI AvrII BbrPI BcgI
BfrI BlnI BsiWI BsmI BspHI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I DraI DrdI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I HindIII
HpaI KspI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2507 GTCTAC GT^[AC][GT]AC
AflIII 2401 ACATGT A^C[GA][CT]GT
Asp700I 1754 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3621 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 244 TGCGCA TGC^GCA
BciVI 2677 GTATCC GTATCC(6/5)
BsaAI 645 TACGTG [CT]AC^GT[GA]
BsaBI 2129 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1632 GAGACG CGTCTC(1/5)
BspDI 4357 ATCGAT AT^CGAT
BspLU11I 2401 ACATGT A^CATGT
BstAPI 380 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 4357 ATCGAT AT^CGAT
CpoI 342 CGGACCG CG^G[AT]CCG
FseI 3171 GGCCGGCC GGCCGG^CC
FspI 244 TGCGCA TGC^GCA
HincII 604 GTCAAC GT[CT]^[GA]AC
HindII 604 GTCAAC GT[CT]^[GA]AC
MamI 2129 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3621 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2532 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 342 CGGACCG CG^G[AT]CCG
ScaI 586 AGTACT AGT^ACT
Tth111I 3621 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1754 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2784 GGTACC G^GTACC
3835 GGTACC
Alw44I 2091 GTGCAC G^TGCAC
2483 GTGCAC
ApaLI 2091 GTGCAC G^TGCAC
2483 GTGCAC
Asp718I 2784 GGTACC G^GTACC
3835 GGTACC
BamHI 989 GGATCC G^GATCC
1387 GGATCC
BbvCI 2057 CCTCAGC CCTCAGC(-5/-2)
2251 CCTCAGC
BclI 214 TGATCA T^GATCA
2569 TGATCA
BglII 1127 AGATCT A^GATCT
1516 AGATCT
BsaI 2208 GAGACC GGTCTC(1/5)
4416 GAGACC
Bsp1407I 2404 TGTACA T^GTACA
4041 TGTACA
BsrBI 2334 CCGCTC CCGCTC(-3/-3)
1857 GAGCGG
BsrDI 3041 CATTGC GCAATG(2/0)
2749 GCAATG
BsrGI 2404 TGTACA T^GTACA
4041 TGTACA
DraIII 381 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4322 CACCCGGTG
EarI 4301 CTCTTC CTCTTC(1/4)
1745 GAAGAG
FbaI 214 TGATCA T^GATCA
2569 TGATCA
KpnI 2784 GGTACC GGTAC^C
3835 GGTACC
Ksp632I 4301 CTCTTC CTCTTC(1/4)
1745 GAAGAG
MslI 1700 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2264 CACGCCCGTG
NheI 788 GCTAGC G^CTAGC
3052 GCTAGC
PaeR7I 783 CTCGAG C^TCGAG
805 CTCGAG
SexAI 382 ACCTGGT A^CC[AT]GGT
1684 ACCTGGT
SfiI 20 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2691 GGCCACATAGGCC
SmaI 2501 CCCGGG CCC^GGG
4026 CCCGGG
SspBI 2404 TGTACA T^GTACA
4041 TGTACA
StuI 3351 AGGCCT AGG^CCT
3379 AGGCCT
Tsp509I 1831 AATT ^AATT
2898 AATT
XbaI 1010 TCTAGA T^CTAGA
2868 TCTAGA
XhoI 783 CTCGAG C^TCGAG
805 CTCGAG
XmaCI 2501 CCCGGG C^CCGGG
4026 CCCGGG
XmaI 2501 CCCGGG C^CCGGG
4026 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII