##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I AspI
AviII BclI BglII BseAI BsiEI
BsiWI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoO65I FbaI
FseI FspI KpnI KspI MfeI
MroI MunI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 4127 CTTAAG C^TTAAG
AhdI 3386 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 17 AGCGCT AGC^GCT
AspEI 3386 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3216 GGATCC G^GATCC
BbeI 3117 GGCGCC GGCGC^C
BcgI 1587 CGAATCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4371 GTATCC GTATCC(6/5)
BfrI 4127 CTTAAG C^TTAAG
BsaBI 2062 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1368 ACCGGA [AT]^CCGG[AT]
BsmBI 3958 GAGACG CGTCTC(1/5)
BsmI 484 GCATTC GAATGC(1/-1)
Bsp1407I 278 TGTACA T^GTACA
BspLU11I 4367 ACATGT A^CATGT
BsrBI 53 CCGCTC CCGCTC(-3/-3)
BsrGI 278 TGTACA T^GTACA
Eam1105I 3386 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 17 AGCGCT AGC^GCT
EcoRV 4458 GATATC GAT^ATC
HindIII 3044 AAGCTT A^AGCTT
HpaI 2815 GTTAAC GTT^AAC
KasI 3117 GGCGCC G^GCGCC
MamI 2062 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 297 ACGCGT A^CGCGT
NaeI 375 GCCGGC GCC^GGC
NarI 3117 GGCGCC GG^CGCC
NgoMIV 375 GCCGGC G^CCGGC
PaeR7I 920 CTCGAG C^TCGAG
PflMI 136 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1315 GAAGAGC GCTCTTC(1/4)
SfoI 3117 GGCGCC GGC^GCC
SmaI 3862 CCCGGG CCC^GGG
SphI 452 GCATGC GCATG^C
SspBI 278 TGTACA T^GTACA
Van91I 136 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2767 TCTAGA T^CTAGA
XhoI 920 CTCGAG C^TCGAG
XmaCI 3862 CCCGGG C^CCGGG
XmaI 3862 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 197 GTAGAC GT^[AC][GT]AC
2461 GTCTAC
Alw44I 3332 GTGCAC G^TGCAC
4278 GTGCAC
ApaLI 3332 GTGCAC G^TGCAC
4278 GTGCAC
Asp700I 3041 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4095 GAATTTTTTC
AvrII 1842 CCTAGG C^CTAGG
3812 CCTAGG
BbrPI 3195 CACGTG CAC^GTG
4275 CACGTG
BbsI 750 GAAGAC GAAGAC(2/6)
2218 GAAGAC
BglI 676 GCCAACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1713 GCCTGTCGGGC
BlnI 1842 CCTAGG C^CTAGG
3812 CCTAGG
BpuAI 750 GAAGAC GAAGAC(2/6)
2218 GAAGAC
BsgI 3364 CTGCAC GTGCAG(16/14)
3823 GTGCAG
BsrDI 1677 CATTGC GCAATG(2/0)
2565 GCAATG
BsrFI 375 GCCGGC [GA]^CCGG[CT]
2346 GCCGGT
Cfr10I 375 GCCGGC [GA]^CCGG[CT]
2346 GCCGGT
EcoRI 1288 GAATTC G^AATTC
2353 GAATTC
EcoT22I 2804 ATGCAT ATGCA^T
3477 ATGCAT
HaeII 17 AGCGCT [GA]GCGC^[CT]
3117 GGCGCC
HincII 2706 GTTGAC GT[CT]^[GA]AC
2815 GTTAAC
HindII 2706 GTTGAC GT[CT]^[GA]AC
2815 GTTAAC
MslI 3470 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3497 CACACCTGTG
NsiI 2804 ATGCAT ATGCA^T
3477 ATGCAT
PmaCI 3195 CACGTG CAC^GTG
4275 CACGTG
PmlI 3195 CACGTG CAC^GTG
4275 CACGTG
PshAI 851 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2539 GACCCTGGTC
StuI 3093 AGGCCT AGG^CCT
3445 AGGCCT
XmnI 3041 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4095 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII