##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BglI BglII
BlnI BsaHI BsaI BsiEI BsiWI
BsmBI BspDI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
CfoI Cfr10I ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FauI FseI
FspI HaeII HhaI Hin1I HinP1I
KasI KpnI KspI MluI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I StuI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 567 TCCGGA T^CCGGA
AciI 3145 CCGC CCGC(-3/-1)
AflII 2700 CTTAAG C^TTAAG
Alw44I 3668 GTGCAC G^TGCAC
AocI 2104 CCTGAGG CC^T[ACGT]AGG
ApaI 426 GGGCCC GGGCC^C
ApaLI 3668 GTGCAC G^TGCAC
AseI 4378 ATTAAT AT^TAAT
AsnI 4378 ATTAAT AT^TAAT
BalI 1665 TGGCCA TGG^CCA
BanII 426 GGGCCC G[GA]GC[CT]^C
BbvCI 1751 GCTGAGG CCTCAGC(-5/-2)
BclI 4304 TGATCA T^GATCA
BcnI 1706 CCGGG CC^[GC]GG
BfrI 2700 CTTAAG C^TTAAG
BsaAI 2746 TACGTA [CT]AC^GT[GA]
BsaBI 966 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 567 TCCGGA [AT]^CCGG[AT]
BseAI 567 TCCGGA T^CCGGA
Bsp1407I 4317 TGTACA T^GTACA
BspEI 567 TCCGGA T^CCGGA
BspHI 3128 TCATGA T^CATGA
BsrGI 4317 TGTACA T^GTACA
BstAPI 1783 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2104 CCTGAGG CC^T[ACGT]AGG
DrdI 1330 GACCAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1665 TGGCCA [CT]^GGCC[GA]
Eco81I 2104 CCTGAGG CC^T[ACGT]AGG
EcoNI 681 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4144 GATATC GAT^ATC
EcoT14I 1241 CCTTGG C^C[AT][AT]GG
FbaI 4304 TGATCA T^GATCA
HincII 1550 GTTAAC GT[CT]^[GA]AC
HindII 1550 GTTAAC GT[CT]^[GA]AC
HindIII 2305 AAGCTT A^AGCTT
HpaI 1550 GTTAAC GTT^AAC
MamI 966 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1203 CAATTG C^AATTG
MluNI 1665 TGGCCA TGG^CCA
MroI 567 TCCGGA T^CCGGA
MscI 1665 TGGCCA TGG^CCA
MunI 1203 CAATTG C^AATTG
NciI 1706 CCGGG CC^[GC]GG
NdeI 2260 CATATG CA^TATG
PaeR7I 1059 CTCGAG C^TCGAG
PflMI 4233 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 540 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4378 ATTAAT AT^TAAT
PstI 91 CTGCAG CTGCA^G
RcaI 3128 TCATGA T^CATGA
SnaBI 2746 TACGTA TAC^GTA
SpeI 3624 ACTAGT A^CTAGT
SspBI 4317 TGTACA T^GTACA
StyI 1241 CCTTGG C^C[AT][AT]GG
Van91I 4233 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1059 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 3668 GTGCAC G[AT]GC[AT]^C
3878 GAGCAC
AvaI 1059 CTCGAG C^[CT]CG[GA]G
1743 CTCGGG
BbsI 3945 GAAGAC GAAGAC(2/6)
3069 GTCTTC
BlpI 1065 GCTCAGC GC^T[ACGT]AGC
2119 GCTGAGC
Bpu1102I 1065 GCTCAGC GC^T[ACGT]AGC
2119 GCTGAGC
BpuAI 3945 GAAGAC GAAGAC(2/6)
3069 GTCTTC
BseRI 465 GAGGAG GAGGAG(10/8)
738 GAGGAG
BsiHKAI 3668 GTGCAC G[AT]GC[AT]^C
3878 GAGCAC
BsoBI 1059 CTCGAG C^[CT]CG[GA]G
1743 CTCGGG
BspLU11I 392 ACATGT A^CATGT
3342 ACATGT
BspMI 1322 ACCTGC ACCTGC(4/8)
341 GCAGGT
Bst1107I 1944 GTATAC GTA^TAC
4656 GTATAC
BstXI 1235 CCATAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1605 CCAGCACTTTGG
BstYI 1346 AGATCC [GA]^GATC[CT]
4507 AGATCC
BstZ17I 1944 GTATAC GTA^TAC
4656 GTATAC
CelII 1065 GCTCAGC GC^T[ACGT]AGC
2119 GCTGAGC
DraII 425 AGGGCCC [GA]G^G[ACGT]CC[CT]
426 GGGCCCT
EcoO109I 425 AGGGCCC [GA]G^G[ACGT]CC[CT]
426 GGGCCCT
EcoT22I 3215 ATGCAT ATGCA^T
4585 ATGCAT
HapII 568 CCGG C^CGG
1706 CCGG
HgaI 1441 GACGC GACGC(5/10)
2924 GACGC
HpaII 568 CCGG C^CGG
1706 CCGG
MflI 1346 AGATCC [GA]^GATC[CT]
4507 AGATCC
MspI 568 CCGG C^CGG
1706 CCGG
NsiI 3215 ATGCAT ATGCA^T
4585 ATGCAT
Tsp45I 847 GTGAC ^GT[GC]AC
4264 GTCAC
XbaI 593 TCTAGA T^CTAGA
3769 TCTAGA
XcmI 526 CCAATAACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1135 CCATTGGATTTGTGG
XhoII 1346 AGATCC [GA]^GATC[CT]
4507 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbvI BciVI BfaI BmyI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI